Title

Supersecretion in Pichia pastoris mutant strains

Poster Number

04B

Lead Author Major

Biological Sciences, Pre-Dentistry

Lead Author Status

Senior

Second Author Major

Biological Sciences

Second Author Status

Junior

Third Author Major

Biological Sciences, Pre-Dentistry

Third Author Status

Junior

Fourth Author Major

Biological Sciences, Pre-Dentistry

Fourth Author Status

Senior

Fifth Author Major

Biological Sciences

Fifth Author Status

Junior

Format

Poster Presentation

Faculty Mentor Name

Joan Lin-Cereghino

Faculty Mentor Email

jlincere@pacific.edu

Faculty Mentor Department

Biological Sciences

Additional Faculty Mentor Name

Geoff Lin-Cereghino

Additional Faculty Mentor Email

glincere@pacific.edu

Additional Faculty Mentor Department

Biological Sciences

Abstract/Artist Statement

Pichia pastoris is a methylotropic yeast that is commonly used for the secretion of large amounts of heterologous protein products. Continuous progress towards increasing secretion of protein products in Pichia pastoris is important for commercial and academic value. While supersecretor mutant strains of Pichia pastoris have been discovered, the mechanism behind supersecretion is unclear. Supersecretion can be attributed to defects in cell wall integrity or to a difference in protein secretion pathway in the mutants. The goal of this project is to use the reporter enzyme Candida antarctica lipase B (CalB) and secretory leukocyte protease inhibitor (SLPI) to test if supersecretion is due to defects in cell wall integrity. For this purpose we have constructed the plasmids pGAPHISαB, pGAPHISαB–CalB and pGAPHISαB-SLPI. The results will allow increased understanding of the mode of supersecretion of the mutant strains.

Location

DeRosa University Center, Ballroom

Start Date

29-4-2017 10:00 AM

End Date

29-4-2017 12:00 PM

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Apr 29th, 10:00 AM Apr 29th, 12:00 PM

Supersecretion in Pichia pastoris mutant strains

DeRosa University Center, Ballroom

Pichia pastoris is a methylotropic yeast that is commonly used for the secretion of large amounts of heterologous protein products. Continuous progress towards increasing secretion of protein products in Pichia pastoris is important for commercial and academic value. While supersecretor mutant strains of Pichia pastoris have been discovered, the mechanism behind supersecretion is unclear. Supersecretion can be attributed to defects in cell wall integrity or to a difference in protein secretion pathway in the mutants. The goal of this project is to use the reporter enzyme Candida antarctica lipase B (CalB) and secretory leukocyte protease inhibitor (SLPI) to test if supersecretion is due to defects in cell wall integrity. For this purpose we have constructed the plasmids pGAPHISαB, pGAPHISαB–CalB and pGAPHISαB-SLPI. The results will allow increased understanding of the mode of supersecretion of the mutant strains.