Title

Small and Large Scale replication of a spider silk protein (MaSp1)

Poster Number

26

Lead Author Major

Biology

Format

Poster Presentation

Faculty Mentor Name

Joan Lin-Cereghino

Faculty Mentor Department

Biological Sciences

Additional Faculty Mentor Name

Geoff Lin-Cereghino

Abstract/Artist Statement

MaSp1 is a protein that can be made happily in the well-known Escherichia coli. This protein can be expressed in small 10mL E. coli cultures, and detected through the process of SDS page electrophoresis. Although micro scale secretion of MaSp1 has been successful, for further studies, mass production of this protein will be needed. We will be using the Bio-Flo 415 Fermentor to test whether or not MaSp1 can be expressed in large quantities.

Location

DeRosa University Center, Ballroom

Start Date

25-4-2015 2:00 PM

End Date

25-4-2015 4:00 PM

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Apr 25th, 2:00 PM Apr 25th, 4:00 PM

Small and Large Scale replication of a spider silk protein (MaSp1)

DeRosa University Center, Ballroom

MaSp1 is a protein that can be made happily in the well-known Escherichia coli. This protein can be expressed in small 10mL E. coli cultures, and detected through the process of SDS page electrophoresis. Although micro scale secretion of MaSp1 has been successful, for further studies, mass production of this protein will be needed. We will be using the Bio-Flo 415 Fermentor to test whether or not MaSp1 can be expressed in large quantities.