Title

Regulation of HDAC6 by Mypt1 in Zebrafish

Poster Number

38

Lead Author Major

Biological Sciences

Format

Poster Presentation

Faculty Mentor Name

Doug Weiser

Faculty Mentor Department

Biological Sciences

Abstract/Artist Statement

Mypt1, myosin phosphatase subunit 1, that regulates protein phosphatase, PP1, by targeting it to myosin and human deacetylase 6. HDAC6 is a histone deacetylase. It deacetylates microtubules, which destabilizes tubulin and decreases alpha and beta tubulin dimerization. Once Mypt1-PP1 targets HDAC6, it dephosphorylates HDAC6, making it inactive. This increases the stabilization of tubulin and ultimately will increase contractibility of the cell. This interaction of proteins is a relatively novel and has only been studied in cell line. We wish to study zebrafish HDAC6 to see if it interacts with Mypt1. To begin this project, we cloned zebrafish HDAC6 into a GFP expression vector. We will next study its protein interaction with Mypt1 using coimmunoprecipitation. We will furthermore investigate the effects of expressing this gene on the cytoskeleton. For future experiments, we will investigate its role in early development in zebrafish.

Location

DeRosa University Center, Ballroom

Start Date

25-4-2015 10:00 AM

End Date

25-4-2015 12:00 PM

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Apr 25th, 10:00 AM Apr 25th, 12:00 PM

Regulation of HDAC6 by Mypt1 in Zebrafish

DeRosa University Center, Ballroom

Mypt1, myosin phosphatase subunit 1, that regulates protein phosphatase, PP1, by targeting it to myosin and human deacetylase 6. HDAC6 is a histone deacetylase. It deacetylates microtubules, which destabilizes tubulin and decreases alpha and beta tubulin dimerization. Once Mypt1-PP1 targets HDAC6, it dephosphorylates HDAC6, making it inactive. This increases the stabilization of tubulin and ultimately will increase contractibility of the cell. This interaction of proteins is a relatively novel and has only been studied in cell line. We wish to study zebrafish HDAC6 to see if it interacts with Mypt1. To begin this project, we cloned zebrafish HDAC6 into a GFP expression vector. We will next study its protein interaction with Mypt1 using coimmunoprecipitation. We will furthermore investigate the effects of expressing this gene on the cytoskeleton. For future experiments, we will investigate its role in early development in zebrafish.