Title

The Function of GADD34 and CReP in the Unfolded Protein Response Pathway and the Downstream Regulation of CHOP

Poster Number

37

Lead Author Major

Pre-Dentistry

Format

Poster Presentation

Faculty Mentor Name

Doug Weiser

Faculty Mentor Department

Biological Sciences

Abstract/Artist Statement

Growth arrest and DNA damage-inducible protein, GADD34, targets protein phosphatase alpha, PP1α, to the endoplasmic reticulum, ER, and dephosphorylates the alpha subunit of the eukaryotic initiation factor 2 (eIF2α). Constitutive repressor of eIF2 phosphorylation, CReP, has homologous protein sequences to GADD34, which means it may have similar functions in targeting PP1α, but CReP is much less studied. Both GADD34 and CReP directly regulate the unfolded protein response (UPR). UPR is a complicated pathway that involves the phosphorylation of eIF2α and the upregulation of the transcription factor, CHOP. All three are a part of a bigger pathway known as the ER stress pathway. Under cell stress, GADD34 and CReP induce cell apoptosis or recovery from stress. How GADD34 and CReP function genetically in this pathway is not well understood, so CHOP can be used as a marker to try and elucidate the roles that both GADD34 and CReP play in vivo. Zebrafish embryos can be used to study these three genes and their regulation of cell apoptosis and homeostasis. Most studies on these three proteins are done in a stressed environment, but we hope to explore their importance in vivo under physiological conditions.

Location

DeRosa University Center, Ballroom

Start Date

25-4-2015 10:00 AM

End Date

25-4-2015 12:00 PM

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Apr 25th, 10:00 AM Apr 25th, 12:00 PM

The Function of GADD34 and CReP in the Unfolded Protein Response Pathway and the Downstream Regulation of CHOP

DeRosa University Center, Ballroom

Growth arrest and DNA damage-inducible protein, GADD34, targets protein phosphatase alpha, PP1α, to the endoplasmic reticulum, ER, and dephosphorylates the alpha subunit of the eukaryotic initiation factor 2 (eIF2α). Constitutive repressor of eIF2 phosphorylation, CReP, has homologous protein sequences to GADD34, which means it may have similar functions in targeting PP1α, but CReP is much less studied. Both GADD34 and CReP directly regulate the unfolded protein response (UPR). UPR is a complicated pathway that involves the phosphorylation of eIF2α and the upregulation of the transcription factor, CHOP. All three are a part of a bigger pathway known as the ER stress pathway. Under cell stress, GADD34 and CReP induce cell apoptosis or recovery from stress. How GADD34 and CReP function genetically in this pathway is not well understood, so CHOP can be used as a marker to try and elucidate the roles that both GADD34 and CReP play in vivo. Zebrafish embryos can be used to study these three genes and their regulation of cell apoptosis and homeostasis. Most studies on these three proteins are done in a stressed environment, but we hope to explore their importance in vivo under physiological conditions.