Title

Manual Synthesis of Polypeptides

Poster Number

43

Lead Author Major

Biochemistry and Biological Sciences

Format

Poster Presentation

Faculty Mentor Name

Jianhua Ren

Faculty Mentor Department

Chemistry

Abstract/Artist Statement

Polypeptides are small chains of amino acid monomers linked together by peptide bonds, which can then be linked together to create longer chains known as polymers or proteins. Enzymes are extremely intricate and complicated structures. They are so complicated that it is highly difficult to study their configuration and function. In place of enzymes, these peptides are used as a substitute to the active site of the enzymes to allow further research to be made. The method known as Solid Phase Peptide Synthesis (SPPS) was used to synthetically make peptides in a laboratory setting. This method requires repeated cycles of deprotection and coupling steps in order to expand the length of the synthesized peptide on a resin such as Rink Amide. Other reactions such as acetylation can be done to further specify the polypeptide. The peptide is then cleaved from the resin using a cleaving reagent. Multiple washing cycles and a final lyophilization were completed to continuously purify the product. A mass spectrometer is used to confirm the identity of the finished product by a full scan and a MS/MS scan. Examples of polypeptides formed include DGG, GGD, Ac-AAAK, Ac-KAAA.

Location

DeRosa University Center, Ballroom

Start Date

26-4-2014 2:00 PM

End Date

26-4-2014 4:00 PM

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Apr 26th, 2:00 PM Apr 26th, 4:00 PM

Manual Synthesis of Polypeptides

DeRosa University Center, Ballroom

Polypeptides are small chains of amino acid monomers linked together by peptide bonds, which can then be linked together to create longer chains known as polymers or proteins. Enzymes are extremely intricate and complicated structures. They are so complicated that it is highly difficult to study their configuration and function. In place of enzymes, these peptides are used as a substitute to the active site of the enzymes to allow further research to be made. The method known as Solid Phase Peptide Synthesis (SPPS) was used to synthetically make peptides in a laboratory setting. This method requires repeated cycles of deprotection and coupling steps in order to expand the length of the synthesized peptide on a resin such as Rink Amide. Other reactions such as acetylation can be done to further specify the polypeptide. The peptide is then cleaved from the resin using a cleaving reagent. Multiple washing cycles and a final lyophilization were completed to continuously purify the product. A mass spectrometer is used to confirm the identity of the finished product by a full scan and a MS/MS scan. Examples of polypeptides formed include DGG, GGD, Ac-AAAK, Ac-KAAA.