The Quest for Super-Secretor Pichia pastoris Strains
Poster Number
16
Format
Poster Presentation
Abstract/Artist Statement
The goal of our project was to create a new mutant library of Pichia pastoris, a yeast commonly used for expressing genes from other species, in order to find new super-secreting strains. The mutagenic plasmid pREMI-Z was randomly inserted into the yeast’s genomic DNA through restriction-enzyme mediated integration (REMI). BamHI was the restriction-enzyme used to mediate this random integration. In order to generate the number of transformants necessary for a complete library, varying amounts of pREMI-Z DNA and BamHI enzyme were tested. After multiple transformations, the optimal conditions for transformation efficiency were determined to be 170 ng of pREMI-Z and 2 units of BamHI.
Location
Pacific Geosciences Center
Start Date
5-5-2007 1:00 PM
End Date
5-5-2007 3:00 PM
The Quest for Super-Secretor Pichia pastoris Strains
Pacific Geosciences Center
The goal of our project was to create a new mutant library of Pichia pastoris, a yeast commonly used for expressing genes from other species, in order to find new super-secreting strains. The mutagenic plasmid pREMI-Z was randomly inserted into the yeast’s genomic DNA through restriction-enzyme mediated integration (REMI). BamHI was the restriction-enzyme used to mediate this random integration. In order to generate the number of transformants necessary for a complete library, varying amounts of pREMI-Z DNA and BamHI enzyme were tested. After multiple transformations, the optimal conditions for transformation efficiency were determined to be 170 ng of pREMI-Z and 2 units of BamHI.