Title

Investigations of the activated B cell factor promoter region

Poster Number

30

Format

Poster Presentation

Abstract/Artist Statement

Activated B cell factor 1 (ABF-l)/MyoR is a basic helix-loop-helix (bHLH) transcriptional repressor that controls B cell proliferation and skeletal muscle differentiation. It has been suggested that the ABF-1 gene is regulated by HES6, another member of the bHLH family. To investigate the role that HES6 plays on the regulation of the ABF-1 gene, we have cloned the mouse promoter region and compared the sequence to the human promoter for conserved regulatory elements. Over a 1.8 kb region, two conserved regulatory elements were identified, both showing over 90% homology within a 70 bp region. In order to determine the potential role of HES6, we have performed a set of transient transfection assays using several different DNA constructs carrying 5' deletions of the human ABF-1 promoter linked to the firefly luciferase reporter gene. The role of HES6 on the regulation of the human ABF-1 promoter will be presented.

Location

Pacific Geosciences Center

Start Date

20-4-2002 9:00 AM

End Date

20-4-2002 5:00 PM

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Apr 20th, 9:00 AM Apr 20th, 5:00 PM

Investigations of the activated B cell factor promoter region

Pacific Geosciences Center

Activated B cell factor 1 (ABF-l)/MyoR is a basic helix-loop-helix (bHLH) transcriptional repressor that controls B cell proliferation and skeletal muscle differentiation. It has been suggested that the ABF-1 gene is regulated by HES6, another member of the bHLH family. To investigate the role that HES6 plays on the regulation of the ABF-1 gene, we have cloned the mouse promoter region and compared the sequence to the human promoter for conserved regulatory elements. Over a 1.8 kb region, two conserved regulatory elements were identified, both showing over 90% homology within a 70 bp region. In order to determine the potential role of HES6, we have performed a set of transient transfection assays using several different DNA constructs carrying 5' deletions of the human ABF-1 promoter linked to the firefly luciferase reporter gene. The role of HES6 on the regulation of the human ABF-1 promoter will be presented.