Comparison of High Fructose and Glucose Consumption on the Impairment of Mesenteric Arterial Function in Female Rats

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FASEB Journal






Supp 1



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High fructose consumption, especially in soft drinks, has shown to contribute to variety of metabolic disorders such as diabetes and obesity. Limited data is available on the relative effects of different dietary sugar intake on vascular reactivity. The objective of this study was to investigate and compare the effects of high glucose (HG) and high fructose (HF) (20% w/v in drinking water for 8 weeks) consumption on functioning of female rat mesenteric arteries (MA). Endothelium-dependent vasodilation (EDV) to acetylcholine (ACh) and bradykinin (BK) and endothelium-independent vasodilation to sodium nitroprusside (SNP) were measured in MA pre-contracted with phenylephrine (PE) or U446619. Furthermore, contractile responses to endothelin-1 (ET-1) were measured. HF ingestion, but not HG, significantly decreased maximal relaxations to ACh and BK. The vasodilation to SNP was impaired in MA from both HF- and HG-fed groups. However, the extent of the impairment was greater in HF compared to HG-fed groups. Finally, the ET-1-induced contraction was augmented in MA of both HG- and HF-fed groups. Our data suggest that a decrease in the sensitivity of smooth muscle to NO may in part contribute to the increased ET-1 contractile responsiveness in HG- and HF-fed groups. Furthermore, the impaired responses to ACh, BK and SNP in MA of HF-fed female rats suggest that the fructose ingestion may have a higher impact in inducing vascular dysfunction compared to glucose ingestion (supported by NIDCR).