Gender difference in the basal intracellular Ca2+ concentration in rat valvular endothelial cells
Biochemical and Biophysical Research Communications
Intracellular Ca concentration ([Ca ](i)) was measured by Fura 2/AM fluorescence imaging microscopy in freshly isolated valvular endothelial cells taken from female and male rats. The basal level of [Ca ]i was significantly elevated in female valvular endothelial cells when compared to males (P < 0.05). Inhibition of the sarco-endoplasmic reticulum Ca -ATPase with cyclopiazonic acid (CPA, 10 μM) caused a greater increase in the [Ca ](i) in female than male endothelial cells. Removal of extracellular Ca returned the [Ca ](i) to the basal level. The rate of [Ca ](i) decline was significantly slower in female endothelial cells compared to males. There were no differences in the unstimulated rate of Mn quenching between two groups. These results demonstrate that estrogen affects NOS at least in part, by an alteration in Ca homeostasis in endothelial cells. 2+ 2+ 2+ 2+ 2+ 2+ 2+ 2+ 2+ 2+
Van Breemen, C.
Gender difference in the basal intracellular Ca2+ concentration in rat valvular endothelial cells.
Biochemical and Biophysical Research Communications, 248(3), 916–919.