The interaction of phospholipid vesicles with black lipid membranes
Nejat Düzgüneş: 0000-0001-6159-1391
Biophysical Society 20th Annual Meeting
February 24–27, 1976
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The electrical properties of black lipid membranes made of phosphatidylserine-decane, incubated in the presence of a suspension of unilamellar phospholipid vesicles J* the aqueous compartment bathing one side of the membrane, are investigated. When Ca is introduced to a phosphatidylcholine vesicle suspension, a simultaneous increase in and fluctuations of the planar membrane are observed. The fluctuations are in steps or spikes over an average base level conductance, which is at least an order of magnitude higher than the initial level. Phosphatidylserine vesicles do not cause such fluctuations, whereas vesicles made of a 1:1 mixture of phosphatidylcholine and phosphatidylserine have an effect similar to ph9phatidylcholine vesicles. The effects of incubation time before the introduction of Ca and the concentration of Ca2+ on the conductance will be reported. The results are discussed in terms of membrane adhesion and fusion, with respect to studies on fusion between spherical bilayers(Breisblatt & Ohki, J. Membrane Biol. 23:385,1975; J. Colloid. Interface Sci., submitted), vesicles (Papahadjopouloss et al., BBA 352:10,1974; BBA 394:483,1975) and between liposomes and planar membranes (Pohl et al., BRA 318:478, 1973).
The interaction of phospholipid vesicles with black lipid membranes.
Paper presented at Biophysical Society 20th Annual Meeting in Seattle, WA.