A nonviral vector with transfection activity comparable with adenoviral transduction

ORCiD

Nejat Düzgüneş: 0000-0001-6159-1391

Department

Biomedical Sciences

Document Type

Article

Publication Title

Therapeutic Delivery

ISSN

2041-5990

Volume

7

Issue

11

DOI

10.4155/tde-2016-0054

First Page

739

Last Page

749

Publication Date

11-1-2016

Abstract

Aim: Viral vectors are used commonly in gene therapy trials, but their potential toxic effects are a serious concern. Identification of highly efficient nonviral vectors may alleviate these effects. Results & methodology: We compared the abilities of TransfeX, TransIT-LT1 and adenovirus to deliver the firefly luciferase and green fluorescent protein genes into HeLa cervical carcinoma, and HSC-3 and H357 oral squamous cell carcinoma cells. TransfeX mediated fourfold higher gene expression in HeLa cells than adenovirus, even at the highest multiplicity of infection. Flow cytometry indicated that a population of transfected cells expresses higher levels of green fluorescent protein than transduced cells. Conclusion: TransfeX may be useful for gene therapy applications, particularly where the use of adenovirus is contraindicated.

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