Title

Gene delivery to oral cancer cells by nonviral vectors: why some cells are resistant to transfection.

ORCiD

Nejat Düzgüneş: 0000-0001-6159-1391

Document Type

Article

Publication Title

Journal of the California Dental Association

ISSN

1043-2256

Volume

37

Issue

12

First Page

855

Last Page

858

Publication Date

1-1-2009

Abstract

The use of cationic lipid-DNA complexes (lipoplexes) for gene therapy of oral squamous cell carcinoma may be limited by the resistance of some cell types to transfection. Using fluorescence microscopy, fluorometry and luciferase luminescence, the authors examined whether the variability arises from the intracellular fate of lipoplexes. In transfection-resistant cells, the efficacy-limiting step appeared to be lipoplex processing beyond binding and internalization, possibly including DNA escape into the cytoplasm and transport into the nucleus.

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