Immunomodulatory and Toxic Effects of Free and Liposome-encapsulated Tumor Necrosis Factor a in Rats1
Nejat Düzgüneş: 0000-0001-6159-1391
Tumor necrosis factor a has potent immunomodulatory and antitumor activity, but its therapeutic applications may be limited by its significant host toxicity. We showed that liposome-encapsulated recombinant human tumor necrosis factor a (rHuTNF-a) retained full anticellular activity in vitro. We then assessed the immunomodulatory and toxic effects of two different doses of i.v. free or liposome-encapsulated rHuTNF-a in normal rats. Both free and liposome-encapsulated rHuTNF-a significantly enhanced alveolar macrophage- and blood monocyte-mediated interleukin 1 release and tumor cell lysis, as well as natural killer cell cytotoxicity, when compared to buffer-treated controls. However, administration of rHuTNF-a in liposomes substantially reduced tumor necrosis factor α-mediated toxicity. Animals receiving liposome-encapsulated rHuTNF-a showed significantly less tissue injury, gastric retention, and circulating leukocyte shifts than animals receiving free rHuTNF-a. In addition, liposome-based delivery significantly increased lung and liver uptake of rHuTNF-a. Therefore, liposome-encapsulated rHuTNF-a retains immunomodulatory activity, significantly reduces toxic inflammatory effects, and may allow targeting of tumor necrosis factor a to selected organs after i.v. administration. © 1990, American Association for Cancer Research. All rights reserved.
Debs, R. J.,
Fuchs, H. J.,
Brunette, E. N.,
Shellito, J. E.,
Patton, J. R.
Immunomodulatory and Toxic Effects of Free and Liposome-encapsulated Tumor Necrosis Factor a in Rats1.
Cancer Research, 50(2), 375–380.