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Date of Award
Thesis - Pacific Access Restricted
Master of Science (M.S.)
First Committee Member
Second Committee Member
Gregg D. Jongeward
The basic helix-loop-helix protein, activated B-cell factor-l (ABF-l), is a transcription factor involved in cellular proliferation and differentiation. ABF-1 shows patterns of expression that are stimulated in activated B cells, which may suggest ABF-1 is involved in the regulation of B-cell differentiation. To investigate the functional role of ABF-1, we have generated a human plasmacytoma cell line with inducible expression of ABF-1. We were able to induce expression of ABF-1 by using the tetracycline repressor system (teton/off). With addition of tetracycline we were able to stimulate the expression of the full-length ABF-1 in the cells. We also got induction of the truncated form of ABF-1, lacking the protein dimerization domain (HLH), with the addition of tetracycline. We then compared these two cell lines to uninduced cells. We confirmed that ABF-1 expression was induced by western blot analysis. We conclude that we have developed a human plasmacytoma cell line with inducible expression of ABF-1 and can use this cell line for further studies.
Fortunati, Jennifer L.. (2004). Development of a human plasmacytoma cell line with inducible expression of activated B-cell factor-1. University of the Pacific, Thesis - Pacific Access Restricted. https://scholarlycommons.pacific.edu/uop_etds/594
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