Campus Access Only

All rights reserved. This publication is intended for use solely by faculty, students, and staff of University of the Pacific. No part of this publication may be reproduced, distributed, or transmitted in any form or by any means, now known or later developed, including but not limited to photocopying, recording, or other electronic or mechanical methods, without the prior written permission of the author or the publisher.

Date of Award

2007

Document Type

Dissertation - Pacific Access Restricted

Degree Name

Doctor of Philosophy (Ph.D.)

Department

Pharmaceutical and Chemical Sciences

First Advisor

Bhaskara Jasti

First Committee Member

Xialing Li

Second Committee Member

Silvio Rodriguez

Third Committee Member

Paul Williams

Fourth Committee Member

Parminder Singh

Fifth Committee Member

Sanjay Goskonda

Abstract

Based on the biochemical composition and structure of the buccal mucosa, drugs can permeate by the lipoidal and/or aqueous pathways. In this regard, the buccal mucosa is similar to skin. As the unionized drug form is the major permeant across skin, flux depends predominantly on the thermodynamic activity of this species. In contrast, ionized drug has been demonstrated to contribute significantly to the permeability across the buccal mucosa due to the presence of large amounts of polar lipids. The contributions of the individual activities of these species is however, not known. Therefore, the first objective of this study was to delineate the thermodynamic activities of ionized and unionized species and to determine their role in governing the total flux across buccal membrane. The flux of model acidic (nimesulide) and basic (bupivacaine) drugs across buccal mucosa either increased (nimesulide) or decreased (bupivacaine) with pH under saturated conditions depending on an increase (nimesulide) or decrease (bupivacaine) in the degree of saturation of ionized species (DS ionized ). At sub-saturated drug concentrations, a decrease in nimesulide flux and an increase in bupivacaine flux were observed with pH due to corresponding changes in DS unionized . For nimesulide and bupivacaine, the contributions of the ionized and unionized species to total flux are equal when 90% of the drug is in the ionized form. In conclusion, the contribution of the ionized form activity to flux was significant. A lack of a specific model for predicting buccal permeability has led to the use of transdermal models such as the Potts-Guy model. However, it is hypothesized that based on the above conclusion, this model might lead to erroneous permeability predictions. In the second part of this dissertation, a specific model was developed and validated by performing permeation studies of 15 small molecules across porcine buccal mucosa. Molecular volume, lipophilicity, number of hydrogen bond donors and number of rotatable bonds were found to be the most significant descriptors governing buccal permeability (logK p ) based on stepwise regression analysis. An excellent fit with an adjusted R 2 of 0.946 and a Q 2 of 0.882 were obtained. A good correlation was observed between the observed and predicted logK p values for an external data set.

Pages

158

ISBN

9780549191988

To access this thesis/dissertation you must have a valid pacific.edu email address and log-in to Scholarly Commons.

Find in PacificSearch Find in ProQuest

Share

COinS

If you are the author and would like to grant permission to make your work openly accessible, please email