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Date of Award
Thesis - Pacific Access Restricted
Master of Science (M.S.)
First Committee Member
Gregg D. Jongeward
Second Committee Member
Third Committee Member
A novel plasma cell-restricted E2A-containing ~-tE2-binding species, designated P-E2A, which forms during the mature B- to plasma cell transition has recently been detected (Jacobs, 1993). In order to elucidate the molecular components of P-E2A, a human plasma cell eDNA library was constructed from the ARH-77 cell line, and the yeast two-hybrid system was employed to search for E2A-interacting plasma cell factors. A hybrid vector that expresses the bHLH region of E4 7 was constructed and utilized as bait to detect E2A-interacting clones. Three clones were isolated that represent possible candidates for in vivo E2A interactions. To improve the specificity and sensitivity of the system, we devised an alternative strategy to the yeast two-hybrid system for the in vivo detection ofE-box-binding E2A-interacting proteins.
Castro, Christopher M.. (1998). The identification of E2A-interacting proteins in human plasma cells : a thesis .... University of the Pacific, Thesis - Pacific Access Restricted. https://scholarlycommons.pacific.edu/uop_etds/2339
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