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Studies on yeasts of medical importance from Stockton, California

Author

Joanna Valencia Cuerpo, University of the Pacific

Date of Award

1992

Document Type

Thesis - Pacific Access Restricted

Degree Name

Master of Science (M.S.)

Department

Graduate School

First Advisor

Fuad M. Nahhas

First Committee Member

Dale McNeal

Second Committee Member

Kishori Chaubal

Abstract

This study deals with the identification of 114 yeast isolates from clinical material collected at Dameron Hospital, Stockton, California between August 15, 1990 and September 16, 1991 . The isolates were identified biochemically using the MicroScan Yeast Identification Panel. All isolates were also tested by four conventional cultural methods: germ tube formation, cycloheximide resistance, chlamydospore production, and spidery colony formation. Biochemically, the yeast isolates were identified to nine species of Candida: C. albicans (ll) , C. tropicalis (8), c. stellatoidea (3), c. guilliermondii (2), C. parapsilosis (2), and one of each species of c. catenulata, C. krusei, C. lusitaniae, and C. pseudotropicalis; one species of Torulopsis: T. glabrata (11) and one species of Kluyveromyces, K. lactis (2). Other than those identified as C. albicans, all the other yeasts identified to the species level were negative for all the four conventional tests except for one of two strains of C. guilliermondii which was positive for germ tube, cycloheximide resistance and chlamydospore production. Based on these three tests this isolate of C. guilliermondii would be identified as C. albicans. Five isolates could not be biochemically identified to species. One of the five was positive for the germ tube, resistance to cycloheximide and chlamydospores. Based solely on these three conventional cultural methods this isolate would be considered c. albicans. This isolate and one of the two strains of C. guilliermondii referred to above raise the question as to a possible shortcoming in the Microscan YIP .

An interesting observation was the finding that all nine strains of C. albicans recovered from stools of different patients belong to the same biotype.

Pages

56

Recommended Citation

Cuerpo, Joanna Valencia. (1992). Studies on yeasts of medical importance from Stockton, California. University of the Pacific, Thesis - Pacific Access Restricted. https://scholarlycommons.pacific.edu/uop_etds/2235