Title

Structure Identification in Complex Mixtures Following Bacterial Settlement Bioactivity via MADByTE NMR-metabolomics Platform

Poster Number

8B

Lead Author Affiliation

Pharmaceutical and Chemical Sciences Program

Lead Author Status

Doctoral Student

Second Author Affiliation

Department of Pharmaceutical Science

Second Author Status

Faculty

Third Author Affiliation

Department of Chemistry

Third Author Status

Faculty

Introduction

Caulerpa spp. are invasive algae that grows in highly competitive tropical and temperate coastal waters that produce many bioactive secondary metabolites. Preliminary data suggests the algal microbiomes of Caulerpa spp. collected in the Florida Keys are chemically mediated.

Purpose

A routine screening of algal extracts on the growth-promotion or -inhibition of 38 Surface-Associated Bacteria (SAB) showed almost no activity. This profound neutral activity led to the development of a novel settlement assay by the Puglisi laboratory used to study the role of chemical mediation in bacterial settlement. This settlement activity was observed in both extracts containing caulerpin and polar extracts that do not contain caulerpin.

Method

Following this unexpected activity, we are investigating the structural features in these complex mixtures that caused settlement of SAB using MADByTE (Metabolomics And Dereplication By Two-Dimensional Experiments) NMR-metabolomics. MADByTE, developed by Dr. Joseph Egan and Dr. Rodger Linnington, uses HSQC and TOCSY experiments to identify similar structural features in complex mixtures with similar biological activities.1 The platform then uses this data to generate molecular networks which then can be integrated with the settlement data.

Results

We aim to identify additional polar and non-polar bioactive molecules responsible for settlement.

Significance

. These results will further guide our study of the role of Caulerpa spp. secondary metabolites in shaping the SAB community.

Location

William Knox Holt Memorial Library and Learning Center, University of the Pacific, 3601 Pacific Ave., Stockton, CA 95211

Format

Poster Presentation

Poster Session

Morning

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Structure Identification in Complex Mixtures Following Bacterial Settlement Bioactivity via MADByTE NMR-metabolomics Platform

William Knox Holt Memorial Library and Learning Center, University of the Pacific, 3601 Pacific Ave., Stockton, CA 95211

Caulerpa spp. are invasive algae that grows in highly competitive tropical and temperate coastal waters that produce many bioactive secondary metabolites. Preliminary data suggests the algal microbiomes of Caulerpa spp. collected in the Florida Keys are chemically mediated.