Separation and Characterization of Cyclopeptides

Poster Number

9C

Lead Author Major

Biological Sciences

Lead Author Status

Senior

Second Author Major

Biochemistry

Second Author Status

Senior

Format

Poster Presentation

Faculty Mentor Name

Skylar Carlson

Faculty Mentor Department

Chemistry

Graduate Student Mentor Name

Savannah Pierce

Graduate Student Mentor Department

Chemistry

Abstract/Artist Statement

Marine cyanobacteria are photosynthetic microbes that often produce compounds that display biological activity and high chemodiversity. In times of stress, these cyanobacteria produce and release many secondary metabolites to help protect themselves and deter predators. Cyclic lipopeptides are closed macro rings that contain a varying number of amino acid residues. Hormothamnion enteromorphoides collected from the Florida Keys is known to produce the cyclic lipopeptide laxaphycin A, a metabolite that exhibits antibacterial and antifungal activity. Laxaphycin A is composed of eleven amino acids, six of which are standard amino acids and five amino acid derivatives, all of which have a different but cohesive role in the overall bioactivity of the lipopeptide. Laxaphycin A is also known for its segregation of hydrophobic and hydrophilic residues to different sides of the overall lipopeptide structure (Darcel et al., 2021). The extracts of H. enteromorphoides were fractionated into five different fractions using bench column chromatography for separation based on polarity. The fractions were then run on the HPLC (high pressure liquid chromatography) to test for UV absorbance and later run on the LCMS (liquid chromatography-mass spectrometry) to analyze the composition of the fraction. We will present our efforts using HPLC and LCMS to isolate and purify laxaphycin A. This isolated metabolite will be sent to Florida for use in a behavioral assay using Porites astreoides coral larvae.

Location

Information Commons, William Knox Holt Memorial Library and Learning Center

Start Date

29-4-2023 10:00 AM

End Date

29-4-2023 1:00 PM

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Apr 29th, 10:00 AM Apr 29th, 1:00 PM

Separation and Characterization of Cyclopeptides

Information Commons, William Knox Holt Memorial Library and Learning Center

Marine cyanobacteria are photosynthetic microbes that often produce compounds that display biological activity and high chemodiversity. In times of stress, these cyanobacteria produce and release many secondary metabolites to help protect themselves and deter predators. Cyclic lipopeptides are closed macro rings that contain a varying number of amino acid residues. Hormothamnion enteromorphoides collected from the Florida Keys is known to produce the cyclic lipopeptide laxaphycin A, a metabolite that exhibits antibacterial and antifungal activity. Laxaphycin A is composed of eleven amino acids, six of which are standard amino acids and five amino acid derivatives, all of which have a different but cohesive role in the overall bioactivity of the lipopeptide. Laxaphycin A is also known for its segregation of hydrophobic and hydrophilic residues to different sides of the overall lipopeptide structure (Darcel et al., 2021). The extracts of H. enteromorphoides were fractionated into five different fractions using bench column chromatography for separation based on polarity. The fractions were then run on the HPLC (high pressure liquid chromatography) to test for UV absorbance and later run on the LCMS (liquid chromatography-mass spectrometry) to analyze the composition of the fraction. We will present our efforts using HPLC and LCMS to isolate and purify laxaphycin A. This isolated metabolite will be sent to Florida for use in a behavioral assay using Porites astreoides coral larvae.