Title

Oral pathogen antimicrobials from a traditional Chinese medicine mushroom: Collaboration between College of the Pacific and Dugoni

Poster Number

18B

Lead Author Major

Biological Sciences

Lead Author Status

Senior

Format

Poster Presentation (Research Day, April 30)

Faculty Mentor Name

Skylar Carlson

Faculty Mentor Department

Chemistry

Graduate Student Mentor Name

Savannah Pierce

Graduate Student Mentor Department

Chemistry

Abstract/Artist Statement

Traditional Chinese Medicine (TCM) has been used for thousands of years and remains the only source of treatment for many less developed areas. Although these practices have been used for so long, there is a major deficit identifying the bioactive constituents of herbal medicines. Ganoderma lucidum, a well known TCM, is a fungus used in Asian countries thought to increase longevity. Recent studies have shown the numerous health benefits which include modulating the immune system, inhibiting bacteria growth, and controlling blood glucose levels (Wachtel-Gator et al. 2011). Preliminary data from the dental school has shown that the methanol and ethanol crude extracts from the fungus inhibit oral pathogens while also not suppressing commensal organism populations. The first step in isolating these compounds is to prepare a chemical extract to remove the small molecules from the mushroom to identify the bioactive components responsible for the observed activity. Using Solid Phase Extraction (SPE) column chromatography, three solvent extractions of G. Lucidum: 100% EtOH, 50:50 EtOH:MeOH, 100% MeOH were fractionated. These fractions are currently being tested against both Gram-positive and Gram-negative human pathogens. Commensal bacterial testing will take place at the Dugoni School of Dentistry.

Location

Information Commons, William Knox Holt Memorial Library and Learning Center

Start Date

30-4-2022 1:00 PM

End Date

30-4-2022 3:00 PM

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Apr 30th, 1:00 PM Apr 30th, 3:00 PM

Oral pathogen antimicrobials from a traditional Chinese medicine mushroom: Collaboration between College of the Pacific and Dugoni

Information Commons, William Knox Holt Memorial Library and Learning Center

Traditional Chinese Medicine (TCM) has been used for thousands of years and remains the only source of treatment for many less developed areas. Although these practices have been used for so long, there is a major deficit identifying the bioactive constituents of herbal medicines. Ganoderma lucidum, a well known TCM, is a fungus used in Asian countries thought to increase longevity. Recent studies have shown the numerous health benefits which include modulating the immune system, inhibiting bacteria growth, and controlling blood glucose levels (Wachtel-Gator et al. 2011). Preliminary data from the dental school has shown that the methanol and ethanol crude extracts from the fungus inhibit oral pathogens while also not suppressing commensal organism populations. The first step in isolating these compounds is to prepare a chemical extract to remove the small molecules from the mushroom to identify the bioactive components responsible for the observed activity. Using Solid Phase Extraction (SPE) column chromatography, three solvent extractions of G. Lucidum: 100% EtOH, 50:50 EtOH:MeOH, 100% MeOH were fractionated. These fractions are currently being tested against both Gram-positive and Gram-negative human pathogens. Commensal bacterial testing will take place at the Dugoni School of Dentistry.