Title

Overcoming Obstacles in Pichia pastoris Protein Expression: The Article and the Movie

Lead Author Major

Biological Sciences B.S.

Lead Author Status

Senior

Second Author Major

Pre-Dentistry

Second Author Status

Senior

Third Author Major

Pre-Dentistry

Third Author Status

Senior

Fourth Author Major

Pre-Dentistry

Fourth Author Status

Senior

Format

Oral Presentation

Faculty Mentor Name

Geoff Lin-Cereghino

Faculty Mentor Department

Department of Biological Sciences

Additional Faculty Mentor Name

Joan Lin-Cereghino

Additional Faculty Mentor Department

Department of Biological Sciences

Abstract/Artist Statement

The yeast Pichia pastoris (also known as Komagataella pastoris) has been used for about 30 years to produce thousands of recombinant proteins, such as insulin to treat diabetes and antibodies to prevent migraines. Despite its success, there are some stubborn problems encountered by research scientists when they try to use Pichia pastoris to produce their recombinant proteins. In order to provide those working in this field with strategies to overcome these common obstacles, we interviewed eight experts in Pichia protein expression to create a written review and video. The article and video contain a collection of short narratives describing how each featured scientist addressed a specific problem, focusing on his/her strategy, key achievements, and the long term effects of the work. The video will be previewed publicly for the first time during this PURCC presentation, and we will be available for any comments and questions. We expect that our work will be a tool to help empower scientists to successfully express challenging proteins in this popular yeast.

Location

University of the Pacific, 3601 Pacific Ave., Stockton, CA 95211

Start Date

24-4-2021 3:45 PM

End Date

24-4-2021 4:00 PM

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Apr 24th, 3:45 PM Apr 24th, 4:00 PM

Overcoming Obstacles in Pichia pastoris Protein Expression: The Article and the Movie

University of the Pacific, 3601 Pacific Ave., Stockton, CA 95211

The yeast Pichia pastoris (also known as Komagataella pastoris) has been used for about 30 years to produce thousands of recombinant proteins, such as insulin to treat diabetes and antibodies to prevent migraines. Despite its success, there are some stubborn problems encountered by research scientists when they try to use Pichia pastoris to produce their recombinant proteins. In order to provide those working in this field with strategies to overcome these common obstacles, we interviewed eight experts in Pichia protein expression to create a written review and video. The article and video contain a collection of short narratives describing how each featured scientist addressed a specific problem, focusing on his/her strategy, key achievements, and the long term effects of the work. The video will be previewed publicly for the first time during this PURCC presentation, and we will be available for any comments and questions. We expect that our work will be a tool to help empower scientists to successfully express challenging proteins in this popular yeast.