Title

Investigation of the Binding of Aminoglycosides to c-Myc G-quadruplex DNA Using the FID Assay

Poster Number

18B

Lead Author Major

Biochemistry

Lead Author Status

Senior

Second Author Major

Biochemistry

Second Author Status

Junior

Third Author Major

Biochemistry

Third Author Status

Sophomore

Format

Poster Presentation

Faculty Mentor Name

Liang Xue

Faculty Mentor Email

lxue@pacific.edu

Faculty Mentor Department

Chemistry

Graduate Student Mentor Name

Vanessa Rangel

Graduate Student Mentor Email

v_rangel1@u.pacific.edu

Graduate Student Mentor Department

Chemistry

Abstract/Artist Statement

More than 300,000 guanine-rich sequences are present in the human genome, many of which can form a unique DNA secondary structure known as G-quadruplex (G4). The formation of G-quadruplex structure has been confirmed in vivo, arousing much attention on their potential structure and function relationship. G-quadruplexes are believed to block the binding of DNA-processing enzymes and subsequently regulate their biological functions. Many small molecules, known as G4 ligands, can facilitate the formation of G-quadruplexes, which have been recognized as potential drugs for the treatment of various diseases. Our previous studies of a class of antibiotics (aminoglycosides) suggested that neomycin can efficiently bind to telomeric G- quadruplex DNA. In this study, we further investigated the binding of aminoglycosides to a G-quadruplex structure formed in the promoter region of an oncogene (c-Myc) using the FID assay. Our results suggest that amongst the nine aminoglycosides tested, neomycin has the best binding affinity toward c-Myc G-quadruplex DNA. The apparent strong binding of neomycin with G-quadruplex DNA probably results from its molecular shape and multiple positive charges.

Location

DeRosa University Center Ballroom

Start Date

27-4-2018 10:00 AM

End Date

27-4-2018 12:00 PM

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Apr 27th, 10:00 AM Apr 27th, 12:00 PM

Investigation of the Binding of Aminoglycosides to c-Myc G-quadruplex DNA Using the FID Assay

DeRosa University Center Ballroom

More than 300,000 guanine-rich sequences are present in the human genome, many of which can form a unique DNA secondary structure known as G-quadruplex (G4). The formation of G-quadruplex structure has been confirmed in vivo, arousing much attention on their potential structure and function relationship. G-quadruplexes are believed to block the binding of DNA-processing enzymes and subsequently regulate their biological functions. Many small molecules, known as G4 ligands, can facilitate the formation of G-quadruplexes, which have been recognized as potential drugs for the treatment of various diseases. Our previous studies of a class of antibiotics (aminoglycosides) suggested that neomycin can efficiently bind to telomeric G- quadruplex DNA. In this study, we further investigated the binding of aminoglycosides to a G-quadruplex structure formed in the promoter region of an oncogene (c-Myc) using the FID assay. Our results suggest that amongst the nine aminoglycosides tested, neomycin has the best binding affinity toward c-Myc G-quadruplex DNA. The apparent strong binding of neomycin with G-quadruplex DNA probably results from its molecular shape and multiple positive charges.