Title

Investigation of Aminoglycosides Binding to G-Quadruplex DNA Using the FID Assay

Poster Number

01B

Lead Author Major

Pre-Pharmacy

Lead Author Status

Senior

Second Author Major

Biochemistry

Second Author Status

Junior

Third Author Major

Biochemistry

Third Author Status

Freshman

Fourth Author Major

Medicine, University of Melbourne

Fourth Author Status

Senior

Format

Poster Presentation

Faculty Mentor Name

Liang Xue

Faculty Mentor Email

lxue@pacific.edu

Faculty Mentor Department

Chemistry

Graduate Student Mentor Name

Siwen Wang

Graduate Student Mentor Email

s_wang13@u.pacific.edu

Graduate Student Mentor Department

Chemistry

Abstract/Artist Statement

The enzyme telomerase is known to maintain the length of chromosomes necessary for cell survival by adding nucleotides to the chromosomal ends; and this enzyme is overexpressed in 80-95% of malignant tumors, conferring cell immortality. The chromosome ends where telomerase acts upon are guanine-rich, which can fold into a DNA secondary structure called G-quadruplex (G4). Interestingly, telomerase cannot bind to G4; therefore, inducing G4 formation at the ends of chromosome regulates telomerase activity. Many small molecules, known as G4 ligands have been developed for stabilizing telomeric G-quadruplex DNA. In the present work, we sought to investigate a class of antibiotics (aminoglycosides) binding to G-quadruplex DNA using an FID assay. Aminoglycosides are known to bind to ribosomal RNA and A-form DNA, but their binding to G-quadruplex DNA remains unclear. Our results suggest that amongst nine aminoglycosides, neomycin has the best binding affinity toward G-quadruplex DNA. The experimental conditions and data analysis will be presented.

Location

DeRosa University Center, Ballroom

Start Date

28-4-2018 1:00 PM

End Date

28-4-2018 3:00 PM

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Apr 28th, 1:00 PM Apr 28th, 3:00 PM

Investigation of Aminoglycosides Binding to G-Quadruplex DNA Using the FID Assay

DeRosa University Center, Ballroom

The enzyme telomerase is known to maintain the length of chromosomes necessary for cell survival by adding nucleotides to the chromosomal ends; and this enzyme is overexpressed in 80-95% of malignant tumors, conferring cell immortality. The chromosome ends where telomerase acts upon are guanine-rich, which can fold into a DNA secondary structure called G-quadruplex (G4). Interestingly, telomerase cannot bind to G4; therefore, inducing G4 formation at the ends of chromosome regulates telomerase activity. Many small molecules, known as G4 ligands have been developed for stabilizing telomeric G-quadruplex DNA. In the present work, we sought to investigate a class of antibiotics (aminoglycosides) binding to G-quadruplex DNA using an FID assay. Aminoglycosides are known to bind to ribosomal RNA and A-form DNA, but their binding to G-quadruplex DNA remains unclear. Our results suggest that amongst nine aminoglycosides, neomycin has the best binding affinity toward G-quadruplex DNA. The experimental conditions and data analysis will be presented.