Title

Synthesis of Oligopeptides for Magnetic Bead Application

Poster Number

09C

Lead Author Major

Pre-Pharmacy

Lead Author Status

Senior

Second Author Major

Biological Sciences

Second Author Status

Sophomore

Format

Poster Presentation

Faculty Mentor Name

Jianhua Ren

Faculty Mentor Email

jren@pacific.edu

Faculty Mentor Department

Chemistry

Graduate Student Mentor Name

Yuntao Zhang

Graduate Student Mentor Email

y_zhang15@u.pacific.edu

Graduate Student Mentor Department

Chemistry

Abstract/Artist Statement

Peptides are short chains of amino acids covalently linked by amide bonds and can be used in many applications. Solid Phase Peptide Synthesis (SPPS) is a preferred method in synthesizing oligopeptides. The versatility of the procedures in a repeated cycle allows for synthesis of a multitude of peptides varying in length and sequence. Because the assembly of the sequence is dependent on an insoluble rink amide resin, excess reagents and side products are easily removed via washes all throughout the procedure. Solid phase peptide synthesis consists of four main parts. The first step is deprotection of the Fmoc protecting group that prevents the amine group from reacting. Second is coupling the appropriate Fmoc protected amino acid in the peptide sequence. Deprotection and coupling are both followed by washes and this cycle is repeated until the desired peptide sequence is completed. The third step is cleavage of the peptide from the resin, resulting in a crude peptide with an amidated C terminus. The fourth is purification and lyophilization of the peptide. One synthesized, the identity of the purified peptide is confirmed through mass spectrometry and product ion analysis. Using this method, we have synthesized several peptides with different amino acid sequences. Specific peptides can be used as functional materials to bind to the surface of magnetic polymer beads to make the beads possess certain functions. Functionalized magnetic beads have a wide variety of applications. In environmental protection for example, they can be used as a sensitive tool to detect toxic materials in water.

Location

DeRosa University Center, Ballroom

Start Date

28-4-2018 1:00 PM

End Date

28-4-2018 3:00 PM

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Apr 28th, 1:00 PM Apr 28th, 3:00 PM

Synthesis of Oligopeptides for Magnetic Bead Application

DeRosa University Center, Ballroom

Peptides are short chains of amino acids covalently linked by amide bonds and can be used in many applications. Solid Phase Peptide Synthesis (SPPS) is a preferred method in synthesizing oligopeptides. The versatility of the procedures in a repeated cycle allows for synthesis of a multitude of peptides varying in length and sequence. Because the assembly of the sequence is dependent on an insoluble rink amide resin, excess reagents and side products are easily removed via washes all throughout the procedure. Solid phase peptide synthesis consists of four main parts. The first step is deprotection of the Fmoc protecting group that prevents the amine group from reacting. Second is coupling the appropriate Fmoc protected amino acid in the peptide sequence. Deprotection and coupling are both followed by washes and this cycle is repeated until the desired peptide sequence is completed. The third step is cleavage of the peptide from the resin, resulting in a crude peptide with an amidated C terminus. The fourth is purification and lyophilization of the peptide. One synthesized, the identity of the purified peptide is confirmed through mass spectrometry and product ion analysis. Using this method, we have synthesized several peptides with different amino acid sequences. Specific peptides can be used as functional materials to bind to the surface of magnetic polymer beads to make the beads possess certain functions. Functionalized magnetic beads have a wide variety of applications. In environmental protection for example, they can be used as a sensitive tool to detect toxic materials in water.