Title

Recombinant expression of the water-clarifying Moringa oleifera cationic protein, MOCP, in the yeast, Pichia pastoris

Poster Number

10B

Lead Author Major

Biological Sciences

Lead Author Status

Senior

Second Author Major

Biological Sciences

Second Author Status

Senior

Format

Poster Presentation

Faculty Mentor Name

Joan Lin-Cereghino

Faculty Mentor Email

jlincere@pacific.edu

Faculty Mentor Department

Biological Sciences

Additional Faculty Mentor Name

Geoff P. Lin-Cereghino

Additional Faculty Mentor Email

glincere@pacific.edu

Additional Faculty Mentor Department

Biological Sciences

Additional Faculty Mentor Name

Mark Brunell

Additional Faculty Mentor Email

mbrunell@pacific.edu

Additional Faculty Mentor Department

Biological Sciences

Abstract/Artist Statement

Crushed seeds of the Moringa oleifera tree are used to purify water in tropical regions of the world. Recently, it has been discovered that the water-clarifying and antibacterial activities in the seeds are associated with a highly-charged protein known as the Moringa oleifera Cationic Protein or MOCP. The yeast, Pichia pastoris, has been used to express large quantities of over 5000 proteins from a variety of organisms. In order to determine whether or not MOCP could be made in greater amounts in this yeast, we tried to clone a genomic MOCP gene directly from plant material. Additionally, we generated a recombinant vector containing an optimized MOCP gene and attempted to express MOCP in small- scale cultures. Expression of MOCP in Pichia pastoris could be the first step in the large-scale production of this important protein so that it can be used by economically disadvantaged individuals who live in places without access to clean water.

Location

DeRosa University Center, Ballroom

Start Date

28-4-2018 10:00 AM

End Date

28-4-2018 12:00 PM

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Apr 28th, 10:00 AM Apr 28th, 12:00 PM

Recombinant expression of the water-clarifying Moringa oleifera cationic protein, MOCP, in the yeast, Pichia pastoris

DeRosa University Center, Ballroom

Crushed seeds of the Moringa oleifera tree are used to purify water in tropical regions of the world. Recently, it has been discovered that the water-clarifying and antibacterial activities in the seeds are associated with a highly-charged protein known as the Moringa oleifera Cationic Protein or MOCP. The yeast, Pichia pastoris, has been used to express large quantities of over 5000 proteins from a variety of organisms. In order to determine whether or not MOCP could be made in greater amounts in this yeast, we tried to clone a genomic MOCP gene directly from plant material. Additionally, we generated a recombinant vector containing an optimized MOCP gene and attempted to express MOCP in small- scale cultures. Expression of MOCP in Pichia pastoris could be the first step in the large-scale production of this important protein so that it can be used by economically disadvantaged individuals who live in places without access to clean water.