Title

A hybrid histidine kinase/response regulator is required for hormogonium development in Nostoc punctiforme

Poster Number

11

Lead Author Major

Biological Sciences

Format

Poster Presentation

Faculty Mentor Name

Doug Risser

Faculty Mentor Department

Biological Sciences

Abstract/Artist Statement

The goal of this project is to identify the genes essential for hormogonium development and motility in the filamentous cyanobacterium Nostoc punctiforme. N. punctiforme differentiates hormogonia, motile filaments which facilitate dispersal, the establishment of nitrogen-fixing symbioses with plants and fungi, and phototaxis. Hormogonium motility is driven by a modified type IV pilus-like system that may also secrete a polysaccharide essential for motility. As part of an ongoing project to identify the genes essential for hormogonium development and motility in N. punctiforme, we are performing an in depth analysis of Npun_R3825, one of several genes identified as essential for hormogonium motility using a transposon mutagenesis screen. Npun_R3825 encodes a hybrid histidine kinase/response regulator, a type of protein that plays a role in signal transduction, allowing bacteria to sense and respond to their environment by activating a phosphorelay. In order to elucidate the role that Npun_R3825 plays in the gene-signaling cascade controlling hormogonium development, a strain with an in-frame deletion of Npun_R3825 was created. This strain was non-motile and failed to undergo any of the morphological changes associated with the development of hormogonia. Based on this data, we speculate that Npun_R3825 plays an essential role in an early stage of hormogonium development.

Location

DeRosa University Center, Ballroom

Start Date

30-4-2016 10:00 AM

End Date

30-4-2016 12:00 PM

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Apr 30th, 10:00 AM Apr 30th, 12:00 PM

A hybrid histidine kinase/response regulator is required for hormogonium development in Nostoc punctiforme

DeRosa University Center, Ballroom

The goal of this project is to identify the genes essential for hormogonium development and motility in the filamentous cyanobacterium Nostoc punctiforme. N. punctiforme differentiates hormogonia, motile filaments which facilitate dispersal, the establishment of nitrogen-fixing symbioses with plants and fungi, and phototaxis. Hormogonium motility is driven by a modified type IV pilus-like system that may also secrete a polysaccharide essential for motility. As part of an ongoing project to identify the genes essential for hormogonium development and motility in N. punctiforme, we are performing an in depth analysis of Npun_R3825, one of several genes identified as essential for hormogonium motility using a transposon mutagenesis screen. Npun_R3825 encodes a hybrid histidine kinase/response regulator, a type of protein that plays a role in signal transduction, allowing bacteria to sense and respond to their environment by activating a phosphorelay. In order to elucidate the role that Npun_R3825 plays in the gene-signaling cascade controlling hormogonium development, a strain with an in-frame deletion of Npun_R3825 was created. This strain was non-motile and failed to undergo any of the morphological changes associated with the development of hormogonia. Based on this data, we speculate that Npun_R3825 plays an essential role in an early stage of hormogonium development.