Title

Factors that induce the transcription and translation of RAD51 in Trichomonas Vaginalis

Poster Number

53

Lead Author Major

Biological Sciences

Format

Poster Presentation

Faculty Mentor Name

Lisa Wrischnik

Faculty Mentor Department

Biological Sciences

Abstract/Artist Statement

The genomic DNA of Trichomonas vaginalis contains a homolog of the recombination protein RAD51. This research set out to investigate the different environmental factors that could induce the expression of this protein. I have isolated the genomic upstream promoter region of the RAD51 sequence and ligated it in front of both Green Flourescent Protein (GFP) and Luciferase cloned into a plasmid (MasterNeo) that can be selected for in Trichomonas. I will transfect these plasmids back into Trichomonas, and so when the Trichomonas vaginalis cells want to produce RAD51, they would produce luciferase or GFP, and glow green. Many different factors will be manipulated in order to test the production of RAD51 under different conditions including, but not limited to: UV irradiation, drugs that damage DNA, changes in the cell cycle, and mixing different strains of Trichomonas together.

Location

DeRosa University Center, Ballroom

Start Date

20-4-2013 1:00 PM

End Date

20-4-2013 3:00 PM

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Apr 20th, 1:00 PM Apr 20th, 3:00 PM

Factors that induce the transcription and translation of RAD51 in Trichomonas Vaginalis

DeRosa University Center, Ballroom

The genomic DNA of Trichomonas vaginalis contains a homolog of the recombination protein RAD51. This research set out to investigate the different environmental factors that could induce the expression of this protein. I have isolated the genomic upstream promoter region of the RAD51 sequence and ligated it in front of both Green Flourescent Protein (GFP) and Luciferase cloned into a plasmid (MasterNeo) that can be selected for in Trichomonas. I will transfect these plasmids back into Trichomonas, and so when the Trichomonas vaginalis cells want to produce RAD51, they would produce luciferase or GFP, and glow green. Many different factors will be manipulated in order to test the production of RAD51 under different conditions including, but not limited to: UV irradiation, drugs that damage DNA, changes in the cell cycle, and mixing different strains of Trichomonas together.