Title

Synthesis of Polypeptides and Cleavage

Poster Number

72

Lead Author Major

Biochemistry

Format

Poster Presentation

Faculty Mentor Name

Jianhua Ren

Faculty Mentor Department

Chemistry

Abstract/Artist Statement

Polypeptides are short polymers that contain amino acid residues and are linked by a peptide bond. The peptide bonds correlate with the secondary structure of proteins, which are either alpha helices or Beta sheets. Changing an amino acid residue in a primary structure can change the way a protein folds; therefore, affecting the function of the protein. In order to understand how the primary structure can affect the protein folding, it is extremely important to understand the chemical properties of polypeptides In this research various peptides are synthesized using the Solid Phase Peptide Synthesis. In this procedure amino acids are attached to Rink Amide resins one at a time until the peptide of choice is synthesized. Then the peptide is cleaved from the resins using a cleavage reagent. In order to obtain a peptide with high yield and high purity, the right cleavage reagent must be used. For Cys-Ala- Ala-Ala and Ala-Ala-Ala-Cys peptides, three cleavage reagents have been tested in our lab: TFA and Reagent R, and Reagent B. The results from the three cleavage reagents will be presented in the poster.

Location

Grave Covell

Start Date

21-4-2012 10:00 AM

End Date

21-4-2012 12:00 PM

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Apr 21st, 10:00 AM Apr 21st, 12:00 PM

Synthesis of Polypeptides and Cleavage

Grave Covell

Polypeptides are short polymers that contain amino acid residues and are linked by a peptide bond. The peptide bonds correlate with the secondary structure of proteins, which are either alpha helices or Beta sheets. Changing an amino acid residue in a primary structure can change the way a protein folds; therefore, affecting the function of the protein. In order to understand how the primary structure can affect the protein folding, it is extremely important to understand the chemical properties of polypeptides In this research various peptides are synthesized using the Solid Phase Peptide Synthesis. In this procedure amino acids are attached to Rink Amide resins one at a time until the peptide of choice is synthesized. Then the peptide is cleaved from the resins using a cleavage reagent. In order to obtain a peptide with high yield and high purity, the right cleavage reagent must be used. For Cys-Ala- Ala-Ala and Ala-Ala-Ala-Cys peptides, three cleavage reagents have been tested in our lab: TFA and Reagent R, and Reagent B. The results from the three cleavage reagents will be presented in the poster.