Title

Effects of MAT alpha deletions on protein secretion in Pichia pastoris

Poster Number

57

Lead Author Major

Biological Sciences

Format

Poster Presentation

Faculty Mentor Name

Geoff Lin-Cereghino

Faculty Mentor Department

Biological Sciences

Additional Faculty Mentor Name

Joan Lin-Cereghino

Abstract/Artist Statement

Pichia pastoris is a yeast known to efficiently express and secrete heterologous proteins. In this yeast, MAT alpha is a signal that can direct protein secretion. Its effect on secretion can be tested using reporter genes, whose protein products can readily be measured. The goal of our project was to create specific deletions in the MAT alpha secretion signal and to determine their effects on secretion. Mutant constructs were made via site-directed mutagenesis, and the reporter genes tested were horseradish peroxidase (HRP) and lipase. We hypothesized that distinct deletions in the MAT alpha secretion signal should affect protein secretion differently, and these effects were tested by comparing secretion of the HRP and lipase proteins.

Location

Grave Covell

Start Date

21-4-2012 10:00 AM

End Date

21-4-2012 12:00 PM

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Apr 21st, 10:00 AM Apr 21st, 12:00 PM

Effects of MAT alpha deletions on protein secretion in Pichia pastoris

Grave Covell

Pichia pastoris is a yeast known to efficiently express and secrete heterologous proteins. In this yeast, MAT alpha is a signal that can direct protein secretion. Its effect on secretion can be tested using reporter genes, whose protein products can readily be measured. The goal of our project was to create specific deletions in the MAT alpha secretion signal and to determine their effects on secretion. Mutant constructs were made via site-directed mutagenesis, and the reporter genes tested were horseradish peroxidase (HRP) and lipase. We hypothesized that distinct deletions in the MAT alpha secretion signal should affect protein secretion differently, and these effects were tested by comparing secretion of the HRP and lipase proteins.