Title

Transmission Electron Microscopy Localization of a Major Cysteien Protease of the Protozoal Parasite Trichomonas vaginalis

Poster Number

47

Lead Author Major

Biological Sciences

Format

Poster Presentation

Faculty Mentor Name

Kirkwood Land

Faculty Mentor Department

Biological Sciences

Additional Faculty Mentor Name

Lisa Wrischnik

Abstract/Artist Statement

We have used transmission electron microscopy to analyze the localization of proteases in trichomonad parasites. In particular, we are interested in the location of a number of different cysteine proteases predicted to be in lysosome- like vesicles. One such protease, CP1, is predicted to have a preprodomain and a predicted transmembrane domain based on genomic analysis. Also, immunofluorescence of trichomonads reveals vesicular localization. Further analysis at the electron microscopic level also reveals vesicular localization. The analysis of these enzymes at the subcellular level will aid in the design of additional experiments to test the role of these parasites in pathogenesis.

Location

Grave Covell

Start Date

21-4-2012 10:00 AM

End Date

21-4-2012 12:00 PM

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Apr 21st, 10:00 AM Apr 21st, 12:00 PM

Transmission Electron Microscopy Localization of a Major Cysteien Protease of the Protozoal Parasite Trichomonas vaginalis

Grave Covell

We have used transmission electron microscopy to analyze the localization of proteases in trichomonad parasites. In particular, we are interested in the location of a number of different cysteine proteases predicted to be in lysosome- like vesicles. One such protease, CP1, is predicted to have a preprodomain and a predicted transmembrane domain based on genomic analysis. Also, immunofluorescence of trichomonads reveals vesicular localization. Further analysis at the electron microscopic level also reveals vesicular localization. The analysis of these enzymes at the subcellular level will aid in the design of additional experiments to test the role of these parasites in pathogenesis.