Title

Examining the Effects of Vitamin D3 on the Attenuation of Rad 51 Protein on Tumor Formation in a Hamster Buccal Pouch Model

Poster Number

24

Format

Poster Presentation

Abstract/Artist Statement

Excessive alcohol and tobacco use have the potential to lead multiple genetic mutations that can result in abnormal cell growth in the upper aerodigestive tract leading to head and neck tumors Vitamin D3 has been shown to suppress the growth of head and neck squanlous cell carcinomas in vitro. In preliminary studies, the Albala has shown that Vitamin D3 can inhibit tumor formation in an animal model of hamster buccal pouch. In a histological study, decreased Rad51 (a DNA repair protein) was found to correlate with human malignancies in lung cancer patients. In this study, we have used a portion of the hamster buccal pouch tissue and tumors that were collected previously and prepared for immunohistochernistry by fixation in formalin to examine the expression of Rad51 in the hamster model after treatment with Vitamin D3. We have cut these fixed tissue samples using a microtome into 4 pm thick sections. The sections were then H & E stained for histological examination of tumors and buccal pouch morphology. The present study aims to extend preliminary findings by identifying the expression of the Rad5 1 in sectioned hamster tissues. We have performed immunohistochemistry using antibodies that detect RadS 1 protein on the buccal pouch tissues samples. Expression of RadS 1 has been examined on both Vitamin D3 treated and untreated hamster cheek pouch sections. Immunocytochemical analysis of the RadS 1 protein was performed using an avidin-biotinperoxidase complex. Our results attempt to correlate the affects of Vitamin D3 on Rad51 in head and neck squamous cell carcinoma.

Location

DeRosa University Center, Ballroom B

Start Date

1-5-2010 1:00 PM

End Date

1-5-2010 3:00 PM

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Examining the Effects of Vitamin D3 on the Attenuation of Rad 51 Protein on Tumor Formation in a Hamster Buccal Pouch Model

DeRosa University Center, Ballroom B

Excessive alcohol and tobacco use have the potential to lead multiple genetic mutations that can result in abnormal cell growth in the upper aerodigestive tract leading to head and neck tumors Vitamin D3 has been shown to suppress the growth of head and neck squanlous cell carcinomas in vitro. In preliminary studies, the Albala has shown that Vitamin D3 can inhibit tumor formation in an animal model of hamster buccal pouch. In a histological study, decreased Rad51 (a DNA repair protein) was found to correlate with human malignancies in lung cancer patients. In this study, we have used a portion of the hamster buccal pouch tissue and tumors that were collected previously and prepared for immunohistochernistry by fixation in formalin to examine the expression of Rad51 in the hamster model after treatment with Vitamin D3. We have cut these fixed tissue samples using a microtome into 4 pm thick sections. The sections were then H & E stained for histological examination of tumors and buccal pouch morphology. The present study aims to extend preliminary findings by identifying the expression of the Rad5 1 in sectioned hamster tissues. We have performed immunohistochemistry using antibodies that detect RadS 1 protein on the buccal pouch tissues samples. Expression of RadS 1 has been examined on both Vitamin D3 treated and untreated hamster cheek pouch sections. Immunocytochemical analysis of the RadS 1 protein was performed using an avidin-biotinperoxidase complex. Our results attempt to correlate the affects of Vitamin D3 on Rad51 in head and neck squamous cell carcinoma.