Title

A microarray experiment to determine the biological activity of metronidazole in Trichomonas

Poster Number

8

Format

Poster Presentation

Faculty Mentor Name

Lisa Wrischnik

Additional Faculty Mentor Name

Kirkwood Land

Abstract/Artist Statement

The mechanism of action of metranidazole, the drug of choice for Trichomoniasis, is largely unknown. The aim of this study is to elucidate the biological activity of this antibiotic. This will be achieved by identifying the specific T. vagina/is genes that the drug acts on. A library ofT. vaginalis genes has been built, and will be used to create eDNA rnicroarray chips. The chips will be used to look at two different strains ofT. vaginal is, one being more virulent than the other. One chip for each strain will be used to compare gene expression in untreated cells versus cells treated with metranidazole. After hybridization of the probe with the chips, they will be analyzed to determine which genes are up-regulated or down-regulated in response to drug treatment. Qnce we have determined which genes are affected, the genes will be sequenced. The function of . the genes will then be determined by the function of homologous genes in other organisms. By identifying the genes that metranidazole regulates, a greater understanding of the drug's mechanism of action will be gained.

Location

Wendell Phillips Center, 1st floor hallways

Start Date

3-5-2008 1:00 PM

End Date

3-5-2008 3:00 PM

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May 3rd, 1:00 PM May 3rd, 3:00 PM

A microarray experiment to determine the biological activity of metronidazole in Trichomonas

Wendell Phillips Center, 1st floor hallways

The mechanism of action of metranidazole, the drug of choice for Trichomoniasis, is largely unknown. The aim of this study is to elucidate the biological activity of this antibiotic. This will be achieved by identifying the specific T. vagina/is genes that the drug acts on. A library ofT. vaginalis genes has been built, and will be used to create eDNA rnicroarray chips. The chips will be used to look at two different strains ofT. vaginal is, one being more virulent than the other. One chip for each strain will be used to compare gene expression in untreated cells versus cells treated with metranidazole. After hybridization of the probe with the chips, they will be analyzed to determine which genes are up-regulated or down-regulated in response to drug treatment. Qnce we have determined which genes are affected, the genes will be sequenced. The function of . the genes will then be determined by the function of homologous genes in other organisms. By identifying the genes that metranidazole regulates, a greater understanding of the drug's mechanism of action will be gained.