Enhanced Secretion in P. pastoris with Maltose Binding Proteins
Poster Number
4
Format
Poster Presentation
Abstract/Artist Statement
To increase the efficiency of protein expression in the yeast Pichia pastoris we have constructed a new plasmid which allows the heterologous protein to be expressed as a fusion with the Maltose Binding Protein (MBP).We have seen that the MBP is easily secreted extracellularly in high yields. With this knowledge we are working on creating a fusion between MBP with other proteins such as FKBP12 and EGFP. The overall goal of this project is to create a fusion partner expression vector teaming up a protein that secretes well extracellularly with a heterologous protein.
Location
Pacific Geosciences Center
Start Date
30-4-2005 1:00 PM
End Date
30-4-2005 3:00 PM
Enhanced Secretion in P. pastoris with Maltose Binding Proteins
Pacific Geosciences Center
To increase the efficiency of protein expression in the yeast Pichia pastoris we have constructed a new plasmid which allows the heterologous protein to be expressed as a fusion with the Maltose Binding Protein (MBP).We have seen that the MBP is easily secreted extracellularly in high yields. With this knowledge we are working on creating a fusion between MBP with other proteins such as FKBP12 and EGFP. The overall goal of this project is to create a fusion partner expression vector teaming up a protein that secretes well extracellularly with a heterologous protein.