Title

Enhanced Secretion in P. pastoris with Maltose Binding Proteins

Poster Number

4

Format

Poster Presentation

Abstract/Artist Statement

To increase the efficiency of protein expression in the yeast Pichia pastoris we have constructed a new plasmid which allows the heterologous protein to be expressed as a fusion with the Maltose Binding Protein (MBP).We have seen that the MBP is easily secreted extracellularly in high yields. With this knowledge we are working on creating a fusion between MBP with other proteins such as FKBP12 and EGFP. The overall goal of this project is to create a fusion partner expression vector teaming up a protein that secretes well extracellularly with a heterologous protein.

Location

Pacific Geosciences Center

Start Date

30-4-2005 1:00 PM

End Date

30-4-2005 3:00 PM

This document is currently not available here.

Share

COinS
 
Apr 30th, 1:00 PM Apr 30th, 3:00 PM

Enhanced Secretion in P. pastoris with Maltose Binding Proteins

Pacific Geosciences Center

To increase the efficiency of protein expression in the yeast Pichia pastoris we have constructed a new plasmid which allows the heterologous protein to be expressed as a fusion with the Maltose Binding Protein (MBP).We have seen that the MBP is easily secreted extracellularly in high yields. With this knowledge we are working on creating a fusion between MBP with other proteins such as FKBP12 and EGFP. The overall goal of this project is to create a fusion partner expression vector teaming up a protein that secretes well extracellularly with a heterologous protein.