Title

Using yeast 2-hybrid analysis to examine the role of the fruit fly gene DmXRCC2 in meiosis

Poster Number

5

Format

Poster Presentation

Abstract/Artist Statement

In mammals, Rad51 is a protein that functions to promote crossovers in meiosis and DNA repair during mitosis. Rad51 utilizes the helper proteins Rad51B, Rad51C, Rad51D, XRCC2, and XRCC3. All these proteins are thought to work together to repair damaged DNA, but it is not known specifically how they interact. In mice, XRCC2 is expressed in testes and might also help in generating crossovers in meiosis. We are attempting to find out how these proteins interact with each other in fruit flies to help us better understand how the Rad51 helpers function in DNA repair and meiosis. Flies have a protein called spindle-A, which is similar to the mammalian Rad51 protein. Flies also have the putative helper proteins spindle-B, spindle-D, DmRad51D, and DmXRCC2, which are similar to the mammalian proteins XRCC3, Rad51C, Rad51D, and XRCC2 respectively. The proteins spindle-B and spindle-D work in meiosis. We want to find out whether the proteins DmRad51D and DmXRCC2 might also function in meiosis. In our experiment, we will be using a 2-hybrid system to see if the following fly proteins interact: spindle-A and DmXRCC2, spindle-B and DmXRCC2, and spindle-D and DmXRCC2. This might tell us whether DmXRCC2 plays a role in meiosis in fruit flies.

Location

Pacific Geosciences Center

Start Date

24-4-2004 9:00 AM

End Date

24-4-2004 5:00 PM

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Apr 24th, 9:00 AM Apr 24th, 5:00 PM

Using yeast 2-hybrid analysis to examine the role of the fruit fly gene DmXRCC2 in meiosis

Pacific Geosciences Center

In mammals, Rad51 is a protein that functions to promote crossovers in meiosis and DNA repair during mitosis. Rad51 utilizes the helper proteins Rad51B, Rad51C, Rad51D, XRCC2, and XRCC3. All these proteins are thought to work together to repair damaged DNA, but it is not known specifically how they interact. In mice, XRCC2 is expressed in testes and might also help in generating crossovers in meiosis. We are attempting to find out how these proteins interact with each other in fruit flies to help us better understand how the Rad51 helpers function in DNA repair and meiosis. Flies have a protein called spindle-A, which is similar to the mammalian Rad51 protein. Flies also have the putative helper proteins spindle-B, spindle-D, DmRad51D, and DmXRCC2, which are similar to the mammalian proteins XRCC3, Rad51C, Rad51D, and XRCC2 respectively. The proteins spindle-B and spindle-D work in meiosis. We want to find out whether the proteins DmRad51D and DmXRCC2 might also function in meiosis. In our experiment, we will be using a 2-hybrid system to see if the following fly proteins interact: spindle-A and DmXRCC2, spindle-B and DmXRCC2, and spindle-D and DmXRCC2. This might tell us whether DmXRCC2 plays a role in meiosis in fruit flies.