Title

Isolation of actin cDNA from Lactodectus Hespersus.

Poster Number

12

Format

Poster Presentation

Abstract/Artist Statement

Actin has a very important function in muscles of many organisms where it is necessary in the cytoskeletal processes of a cell. We have attempted to clone the actin cDNA from the black widow spider, Lactodectus Hesperus. This was done by first designing primers based on evolutionarily conserved regions of the actin gene of various organisms. These primers found and extracted a fragment, by Polymerase Chain Reaction (PCR), which matched a predicted size. The authenticity of the fragment will later be confirmed at the nucleotide level by DNA sequencing. We are interested in cloning the black widow's actin cDNA for a number of reasons. First of all because of it's use as a normalizing agent. Actin is useful at quantization of RNA's in tissue sample comparisons because it is known to have relatively constant concentrations in most tissues. Another use of this cloned sequence will be for comparison of conservation in structure and sequence amongst organisms.

Location

Pacific Geosciences Center

Start Date

26-4-2003 9:00 AM

End Date

26-4-2003 5:00 PM

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Apr 26th, 9:00 AM Apr 26th, 5:00 PM

Isolation of actin cDNA from Lactodectus Hespersus.

Pacific Geosciences Center

Actin has a very important function in muscles of many organisms where it is necessary in the cytoskeletal processes of a cell. We have attempted to clone the actin cDNA from the black widow spider, Lactodectus Hesperus. This was done by first designing primers based on evolutionarily conserved regions of the actin gene of various organisms. These primers found and extracted a fragment, by Polymerase Chain Reaction (PCR), which matched a predicted size. The authenticity of the fragment will later be confirmed at the nucleotide level by DNA sequencing. We are interested in cloning the black widow's actin cDNA for a number of reasons. First of all because of it's use as a normalizing agent. Actin is useful at quantization of RNA's in tissue sample comparisons because it is known to have relatively constant concentrations in most tissues. Another use of this cloned sequence will be for comparison of conservation in structure and sequence amongst organisms.