Title

Construction and screening of new EGFP-PTSI1 vectors in pichia pastoris

Poster Number

24

Format

Poster Presentation

Abstract/Artist Statement

EGFP-PTS1 is a fusion of the gene for enhanced green fluorescent protein and a peroxisome targeting sequence. Originally this complex was inserted into a vector with zeocin as a selectable marker. Zeocin is an expensive antibiotic to work with. Amino acids such as histidine and adenine are less expensive to use. In order to generate a similar vector based on amino acid auxotrophy, the EGFP-PTS1 gene will be removed from the zeocin vector and inserted into vectors selecting for either adenine or histidine. Through different screening techniques I hope to determine which new complexes are accurate and functioning.

Location

Pacific Geosciences Center

Start Date

20-4-2002 9:00 AM

End Date

20-4-2002 5:00 PM

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Apr 20th, 9:00 AM Apr 20th, 5:00 PM

Construction and screening of new EGFP-PTSI1 vectors in pichia pastoris

Pacific Geosciences Center

EGFP-PTS1 is a fusion of the gene for enhanced green fluorescent protein and a peroxisome targeting sequence. Originally this complex was inserted into a vector with zeocin as a selectable marker. Zeocin is an expensive antibiotic to work with. Amino acids such as histidine and adenine are less expensive to use. In order to generate a similar vector based on amino acid auxotrophy, the EGFP-PTS1 gene will be removed from the zeocin vector and inserted into vectors selecting for either adenine or histidine. Through different screening techniques I hope to determine which new complexes are accurate and functioning.