Title

The expression of human FKBP12 gene in Pichia Pastoris

Poster Number

22

Format

Poster Presentation

Abstract/Artist Statement

Recent studies have suggested that the regulatory protein, FKBP12 plays a role in T lymphocyte reproduction via the calcium influx pathway. Improper functioning of FKBP12 could lead to uncontrolled T lymphocyte production, which is responsible for T lymphocyte cancers. To enable Dr. David Thomas of the University of the Pacific Dept. of Physiology and Pharmacology to explore how FKBP12 affects calcium dependent growth decisions of cells in the immune system; production of a large amount of the protein is necessary. The yeast, Pichia pastoris has the ability to express proteins from other organisms at high levels. Thus, once the gene coding for FKBP12 was deduced, polymerase chain reaction (PCR) was used to amplify the gene. The gene was then inserted into two different expression vectors for Pichia pastoris. The vector, pPICZα has gene secretion capabilities and the other strain, pPICZ does not have this ability. The most efficient vector will be used to synthesize the protein that Dr. Thomas will use in his cancer research.

Location

Pacific Geosciences Center

Start Date

20-4-2002 9:00 AM

End Date

20-4-2002 5:00 PM

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Apr 20th, 9:00 AM Apr 20th, 5:00 PM

The expression of human FKBP12 gene in Pichia Pastoris

Pacific Geosciences Center

Recent studies have suggested that the regulatory protein, FKBP12 plays a role in T lymphocyte reproduction via the calcium influx pathway. Improper functioning of FKBP12 could lead to uncontrolled T lymphocyte production, which is responsible for T lymphocyte cancers. To enable Dr. David Thomas of the University of the Pacific Dept. of Physiology and Pharmacology to explore how FKBP12 affects calcium dependent growth decisions of cells in the immune system; production of a large amount of the protein is necessary. The yeast, Pichia pastoris has the ability to express proteins from other organisms at high levels. Thus, once the gene coding for FKBP12 was deduced, polymerase chain reaction (PCR) was used to amplify the gene. The gene was then inserted into two different expression vectors for Pichia pastoris. The vector, pPICZα has gene secretion capabilities and the other strain, pPICZ does not have this ability. The most efficient vector will be used to synthesize the protein that Dr. Thomas will use in his cancer research.