Title

Identification of novel ABF-1 interacting proteins using the yeast 2-hybrid system.

Poster Number

6

Format

Poster Presentation

Abstract/Artist Statement

Basic Helix-loop-Helix (bHLH) proteins are involved in cellular differentiation and proliferation. Recently, a bHLH tissue-restricted protein, known as activated B-cell factor (ABF-1) has been identified. To date, ABF-1 represents the only B -cellrestricted protein identified. ABF-1 is expressed.in B-cells treated with mitogen, suggesting a role in B-cell proliferation. In addition elevated levels of ABF-1 are displayed in B-cells infected with Epstein-Barr virus (EBV), an agent known to immortalize B-cells. In order to identify novel protein interactions with ABF-1 a eDNA library, isolated from EBV transformed B-cells, was screened using the yeast 2-hybrid system. The yeast 2-hybrid system is a technique commonly employed to identify protein interactions in vivo. The results obtained from this screening procedure will ];)e discussed.

Location

DeRosa University Center

Start Date

1-5-2001 9:00 AM

End Date

1-5-2001 5:00 PM

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May 1st, 9:00 AM May 1st, 5:00 PM

Identification of novel ABF-1 interacting proteins using the yeast 2-hybrid system.

DeRosa University Center

Basic Helix-loop-Helix (bHLH) proteins are involved in cellular differentiation and proliferation. Recently, a bHLH tissue-restricted protein, known as activated B-cell factor (ABF-1) has been identified. To date, ABF-1 represents the only B -cellrestricted protein identified. ABF-1 is expressed.in B-cells treated with mitogen, suggesting a role in B-cell proliferation. In addition elevated levels of ABF-1 are displayed in B-cells infected with Epstein-Barr virus (EBV), an agent known to immortalize B-cells. In order to identify novel protein interactions with ABF-1 a eDNA library, isolated from EBV transformed B-cells, was screened using the yeast 2-hybrid system. The yeast 2-hybrid system is a technique commonly employed to identify protein interactions in vivo. The results obtained from this screening procedure will ];)e discussed.