Title

Protein Composition of web silk in LATRODECTUS HESPERUS and pholcids

Poster Number

10

Format

Poster Presentation

Abstract/Artist Statement

Latrodectus hesperus and Pholcids (species unknown) are both cob weaving spiders whose silk proteins can be examined through the use of SDS polyacrylamide gel. In order to do this, silk was collected and dissolved in guanidine. Once the silk dissolved, the guanidine was removed using dialysis tubes and the samples were run on a 2-15% gel. A protein gel helps identify proteins by separating them by size. The larger proteins do not run very far on the gel while the smaller ones end up towards the bottom. Using a marker with proteins of known sizes, the sizes of the samples can be determined and also compared to other samples. Because the two spiders come from completely different ancestors but share similarities in web structure, running a gel could help find whether the proteins are identical or different.

Location

DeRosa University Center

Start Date

1-5-2001 9:00 AM

End Date

1-5-2001 5:00 PM

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Protein Composition of web silk in LATRODECTUS HESPERUS and pholcids

DeRosa University Center

Latrodectus hesperus and Pholcids (species unknown) are both cob weaving spiders whose silk proteins can be examined through the use of SDS polyacrylamide gel. In order to do this, silk was collected and dissolved in guanidine. Once the silk dissolved, the guanidine was removed using dialysis tubes and the samples were run on a 2-15% gel. A protein gel helps identify proteins by separating them by size. The larger proteins do not run very far on the gel while the smaller ones end up towards the bottom. Using a marker with proteins of known sizes, the sizes of the samples can be determined and also compared to other samples. Because the two spiders come from completely different ancestors but share similarities in web structure, running a gel could help find whether the proteins are identical or different.