Lipid-Coated, pH-Sensitive Magnesium Phosphate Particles for Intracellular Protein Delivery
PURPOSE: To develop cationic lipid-coated magnesium phosphate nanoparticles (LPP) for intracellular catalase (CAT) delivery.
METHODS: Magnesium phosphate nanoparticles (MgP NP) were prepared by micro-emulsion precipitation and mixed with catalase-loaded cationic liposomes (DOTAP/cholesterol) to yield LPP formulation of catalase (LPP-CAT). The size and ζ-potential of LPP-CAT were measured by dynamic light scattering. The pH-sensitivity of LPP-CAT was determined by monitoring their degradation of hydrogen peroxide (H
RESULTS: LPP-CAT were successfully prepared and carried an average diameter ofζ -potential of about +40 mV. At pH 5.5, LPP-CAT degraded H
CONCLUSION: LPP represents a promising nano-system for intracellular protein delivery.
Lipid-Coated, pH-Sensitive Magnesium Phosphate Particles for Intracellular Protein Delivery.
Pharmaceutical Research, 36(6), 81–81.