Lead Author Affiliation

UOP Graduate Orthodontic Department

Lead Author Status

Masters Student

Presentation Category

Orthodontics Resident Presentations

Introduction/Context/Diagnosis

Endoplasmic reticulum (ER) stress is involved in the pathogenesis of congenital cardiac defects, intrauterine growth restriction, craniofacial anomalies, and many common diseases. The purpose of this study was to test if folic acid (FA) can mitigate ER stress or improve recovery of cells from ER stress.

Methods/Treatment Plan

Human dental pulp stem cells (HDPSC, Celprogen) were cultured in the presence or absence of folate (Molecular Products). Alpha MEM cultivation medium was supplemented with 10% human serum, L-glutamine, and penicillin/streptomycin mixture. Folic acid concentrations were 0.002 µg/ml, 0.2 µg/ml, 2 µg/ml, and 20 µg/ml. Cells were grown in triplicates in atmospheric oxygen and in 0.5% oxygen (Biopherix chamber) with adequate controls.

Results/Outcome

HDPSC were cultivated in 0.5% oxygen for 1, 2, 4, and 6 hours. An optimal timing was determined. HDPSC were cultivated in different concentrations of FA in atmospheric oxygen for 3 days. Compared to controls, no difference in cell counts was observed. HDPSC were subsequently exposed to 0.5% oxygen for two hours in different concentrations of folic acid. A significant decrease in the number of dead cells was found when HDPSC were maintained in a medium containing 2 µg/ml or 20 µg/ml of folic acid.

Significance/Conclusions

FA maintained the viability of approximately half of HDPSC in 0.5% oxygen for two hours. This study will enable us to better understand the underlying molecular mechanisms and processes attenuating ER stress.

Location

2nd floor clinic and reception waiting areas

Format

Poster

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May 23rd, 2:00 PM May 23rd, 4:15 PM

Effect of Folate on Endoplasmic Reticulum Stress Caused by Hypoxia

2nd floor clinic and reception waiting areas

Endoplasmic reticulum (ER) stress is involved in the pathogenesis of congenital cardiac defects, intrauterine growth restriction, craniofacial anomalies, and many common diseases. The purpose of this study was to test if folic acid (FA) can mitigate ER stress or improve recovery of cells from ER stress.