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Identification and characterization of BEN1, a novel microtubule associated protein in fission yeast

Author

Benjamin Alan Escotto, University of the Pacific

Date of Award

2009

Document Type

Thesis - Pacific Access Restricted

Degree Name

Master of Science (M.S.)

Department

Biological Sciences

First Advisor

Srinivas Venkatram

First Committee Member

Geoff Lin-Cereghino

Second Committee Member

Joan Lin-Cereghino

Third Committee Member

Craig A. Vierra

Abstract

In the fission yeast S. pombe, Mto1p and Mto2p are involved in MT nucleation from cytoplasmic MTOCs and recruit components of the γ-TuC. We wished to discover whether Ben1p plays a similar role. ben1⁺ was tagged at its normal chromosomal location and the intracellular localization was analyzed. It showed that Ben1p localized to cytoplasmic microtubules. We generated ben1Δ. cells by replacing it with a copy of ura4⁺. We observed that 20% of the cells were undergoing division. Next we observed the effects that deleting ben1⁺ would have on the functions of Mto1p and Mto2p. We setup crosses between these strains and observed their localization. We did not observe any specific Mto2-GFP localization in ben1Δ. cells. This suggests that Ben1p plays a role in the proper localization of a γ-TuC associated protein.

Pages

61

Recommended Citation

Escotto, Benjamin Alan. (2009). Identification and characterization of BEN1, a novel microtubule associated protein in fission yeast. University of the Pacific, Thesis - Pacific Access Restricted. https://scholarlycommons.pacific.edu/uop_etds/715