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Development of a human plasmacytoma cell line with inducible expression of activated B-cell factor-1
Date of Award
2004
Document Type
Thesis - Pacific Access Restricted
Degree Name
Master of Science (M.S.)
Department
Biological Sciences
First Advisor
Craig Vierra
First Committee Member
Lisa Wrischnik
Second Committee Member
Gregg D. Jongeward
Abstract
The basic helix-loop-helix protein, activated B-cell factor-l (ABF-l), is a transcription factor involved in cellular proliferation and differentiation. ABF-1 shows patterns of expression that are stimulated in activated B cells, which may suggest ABF-1 is involved in the regulation of B-cell differentiation. To investigate the functional role of ABF-1, we have generated a human plasmacytoma cell line with inducible expression of ABF-1. We were able to induce expression of ABF-1 by using the tetracycline repressor system (teton/off). With addition of tetracycline we were able to stimulate the expression of the full-length ABF-1 in the cells. We also got induction of the truncated form of ABF-1, lacking the protein dimerization domain (HLH), with the addition of tetracycline. We then compared these two cell lines to uninduced cells. We confirmed that ABF-1 expression was induced by western blot analysis. We conclude that we have developed a human plasmacytoma cell line with inducible expression of ABF-1 and can use this cell line for further studies.
Pages
46
Recommended Citation
Fortunati, Jennifer L.. (2004). Development of a human plasmacytoma cell line with inducible expression of activated B-cell factor-1. University of the Pacific, Thesis - Pacific Access Restricted. https://scholarlycommons.pacific.edu/uop_etds/594
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