Tissue culture of Camptotheca acuminata decaisne (Nyssaceae)

Author

Ron Charles Cooke, University of the Pacific

Date of Award

1973

Document Type

Thesis

Degree Name

Master of Science (M.S.)

Department

Biological Sciences

First Advisor

John K. Brown

First Committee Member

Herschel Frye

Second Committee Member

Madhukar Chaubal

Third Committee Member

Emmons E. Roscoe

Fourth Committee Member

Donald M. Pace

Abstract

Camptotheca acuminata Decaisne (Nyssaceae) is endemic to China. The plant was brought to the United States only on a few occasions and thus can be considered rare in this country. A search of the plant kingdom for species that produced anticancer substances revealed that ethanolic extracts of Camptotheca acuminata had such a property. The active constituent was identified eventually. Further testing and possible marketing of the active principle, and alkaloid, (camptothecin) depended on a combination of factors: obtaining the plant from China, organization of a mass planting program in the United States or chemical synthesis of the drug.

Due to the dissention between China and the United States during this period, (1950-1960’s) the first alternative was ruled out. Planting programs were organized but the plant needs a relatively long time to mature. Attempts to synthesize camptothecin were started but success was not immediate and there was no guarantee of ever achieving synthesis.

As an alternative to these methods, the author of this research proposed an in vitro system of obtaining the drug. The plant would be grown in culture and the active principle extracted.

The complete story of camptothecin, the rationale for in vitro culture in general and the methods and results of this project are given in the following chapters.

Pages

68

Recommended Citation

Cooke, Ron Charles. (1973). Tissue culture of Camptotheca acuminata decaisne (Nyssaceae). University of the Pacific, Thesis. https://scholarlycommons.pacific.edu/uop_etds/1799