Title

Magnetic resonance microscopy for monitoring osteogenesis in tissue-engineered construct in vitro.

Document Type

Article

Publication Title

Physics in Medicine and Biology

ISSN

0031-9155

Volume

51

Issue

3

DOI

10.1088/0031-9155/51/3/016

First Page

719

Last Page

732

Publication Date

2-7-2006

Abstract

Magnetic resonance microscopy (MRM) is used to monitor osteogenesis in tissue-engineered constructs. Measurements of the developing tissue's MR relaxation times (T(1) and T(2)), apparent diffusion coefficient (ADC) and elastic shear modulus were conducted over a 4-week growth period using an 11.74 T Bruker spectrometer with an imaging probe adapted for MR elastography (MRE). Both the relaxation times and the ADC show a statistically significant decrease after only one week of tissue development while the tissue stiffness increases progressively during the first two weeks of in vitro growth. The measured MR parameters are correlated with histologically monitored osteogenic tissue development. This study shows that MRM can provide quantitative data with which to characterize the growth and development of tissue-engineered bone.