Title

Comparison of high fructose and glucose consumption on the impairment of mesenteric arterial function in female rats

Poster Number

25

Lead Author Affiliation

Physiology and Pharmacology

Introduction

There is an increased consumption of fructose, primarily from high fructose corn syrup (HFCS), a major sweetener in food and soft drinks. High fructose consumption might be one of the contributing factors to the occurrence of metabolic syndrome. However, limited data is available on the relative effects of different dietary sugar intake on vascular reactivity.

Purpose

The objective of this study was to investigate and compare the effects of high glucose (HG) and high fructose (HF) (20% w/v in drinking water for 8 weeks) consumption on functioning of female rat mesenteric arteries (MA).

Method

Sprague-Dawley female rats were supplemented with 20% w/v glucose or fructose in drinking water for 8 weeks. Control rats received drinking water without sugar supplement. Body weight, food and drink intake were monitored throughout the experiment. Endothelium-dependent vasodilation (EDV) to acetylcholine (ACh, 10-8 to 10-5M) and bradykinin (BK, 10-9 to 10-5M) and endothelium-independent vasodilation to sodium nitroprusside (SNP, 10-9 to 10-5M) were measured in pre-contracted MA with phenylephrine (PE, 2 μM) or U446619 (100 nM). Furthermore, contractile responses to endothelin-1 (ET-1, 10-10 to 10-7M ) were also measured.

Results

HF and not HG ingestion increased body weight despite high caloric intake in HG group. HF ingestion, but not HG, significantly decreased maximal relaxations to ACh and BK. The vasodilation to SNP was impaired in MA from both HF- and HG-fed groups. However, the extent of the impairment was greater in HF compared to HG-fed groups. Finally, the ET-1-induced contraction was augmented in MA of both HG- and HF-fed groups.

Significance

Our data suggests that a decrease in the sensitivity of smooth muscle to NO may in part contribute to the increased ET-1 contractile responsiveness in HG- and HF-fed groups. Furthermore, the impaired responses to ACh, BK and SNP in MA of HF-fed female rats suggest that the fructose ingestion may have a higher impact in inducing vascular dysfunction compared to glucose ingestion.

Location

DeRosa University Center, Stockton campus, University of the Pacific

Format

Poster Presentation

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Apr 25th, 10:00 AM Apr 25th, 12:00 PM

Comparison of high fructose and glucose consumption on the impairment of mesenteric arterial function in female rats

DeRosa University Center, Stockton campus, University of the Pacific

There is an increased consumption of fructose, primarily from high fructose corn syrup (HFCS), a major sweetener in food and soft drinks. High fructose consumption might be one of the contributing factors to the occurrence of metabolic syndrome. However, limited data is available on the relative effects of different dietary sugar intake on vascular reactivity.