Title

Expression of human Aryl Hydrocarbon Receptor (AhR) using Pichia pastoris

Poster Number

19

Lead Author Affiliation

Pharmaceutics and medicinal chemistry

Introduction

Pichia is a species of methylotropic yeast. It can use methanol as its carbon and energy source. Pichia pastoris is a widely used host organism for expressing of heterologous protein through recombinant DNA technique due to its low cost and high efficiency.

Purpose

Express human AhR protein by Pichia pastoris cells.

Method

PICZ-B plasmid carrying Pichia optimized human AhR cDNA was transformed into Pichia cells, followed by incubation in glucose media until OD 6 to 9 was reached. Methanol was used to induce protein expression. Affinity purification was performed to enrich recombinant AhR protein. Coomassie blue staining and western blot analysis were performed.

Results

Pichia pastoris can express human AhR protein successfully. The protein expression level using the Pichia pastoris cells were comparable with baculovirus AhR expression.

Significance

Pichia overexpression of human AhR is a viable approach to express human AhR for functional studies.

Location

DeRosa University Center, Stockton campus, University of the Pacific

Format

Poster Presentation

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Expression of human Aryl Hydrocarbon Receptor (AhR) using Pichia pastoris

DeRosa University Center, Stockton campus, University of the Pacific

Pichia is a species of methylotropic yeast. It can use methanol as its carbon and energy source. Pichia pastoris is a widely used host organism for expressing of heterologous protein through recombinant DNA technique due to its low cost and high efficiency.