Clathrin and synaptic vesicle endocytosis: studies at the squid giant synapse

ORCiD

0000-0002-6489-4651

Document Type

Article

Publication Title

Biochemical Society Transactions

ISSN

0300-5127

Volume

34

Issue

Pt 1

DOI

10.1042/BST0340068

First Page

68

Last Page

72

Publication Date

2-1-2006

Abstract

The role of clathrin-mediated endocytosis in SV (synaptic vesicle) recycling has been studied by combining molecular biology, physiology and electron microscopy at the squid giant synapse. Procedures that prevent clathrin from assembling into membrane coats, such as impairment of binding of the AP180 and AP-2 adaptor proteins, completely prevent membrane budding during endocytosis. These procedures also reduce exocytosis, presumably an indirect effect of a reduction in the number of SVs following block of endocytosis. Disrupting the binding of auxilin to Hsc70 (heat-shock cognate 70) prevents clathrin-coated vesicles from uncoating and also disrupts SV recycling. Taken together, these results indicate that a clathrin-dependent pathway is the primary means of SV recycling at this synapse under physiological conditions.

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