Title

Overexpression, purification and characterization of a recombinant secretary catalase from Bacillus subtilis

Document Type

Article

Publication Title

Biotechnology Letters

ISSN

0141-5492

Volume

30

Issue

1

DOI

10.1007/s10529-007-9510-7

First Page

181

Last Page

186

Publication Date

1-1-2008

Abstract

A recombinant Bacillus subtilis strain (KN25) was generated for the large-scale preparation of catalase. The B. subtilis katA gene encoding for catalase was cloned into the shuttle vector PRB374, downstream of the constitutively active vegII promoter, followed by transformation of the B. subtilis strain WB600 with the plasmid. The transformant strain, KN25 secretes high levels (3,500 U/ml) of catalase, which facilitates its purification. Three simple purification steps yielded nearly homogeneous catalase, with approximately 70% recovery. The purified recombinant catalase has a specific activity of 34,600 U/mg under optimal conditions, and is more resistant to acidic conditions than bovine liver catalase.