Presentation Category
Research
Introduction/Context/Diagnosis
Despite advances in dental care and oral hygiene, the prevalence of chronic periodontitis (ChP) remains high at 47.2% in the U.S. ChP not only leads to severe gum bleeding and tooth loss but also contributes to systemic diseases, including diabetes mellitus, adverse pregnancy outcomes, and cardiovascular diseases. The mechanisms of the connection between ChP and systemic health are still not fully understood. In our phase I study, we demonstrated systemic and cell-specific immune dysfunctions in patients with ChP, which can be temporarily reversed by the local treatment of ChP. For phase II study, we aim to 1) validate our findings with larger cohorts across wider time range and 2) investigate ChP oral and systemic immunological interaction by a multiplex cross-tissue analysis. Whole-blood samples from 16 patients with ChP and 12 healthy controls without ChP were collected at baseline (n=28), 3 weeks post-ChP treatment (n= 25), and 3 months post-ChP treatment (n= 25) in the Bell Dental Center (San Leandro, CA). The blood samples were left unstimulated or stimulated with Porphyromonas gingivalis lipopolysaccharide (PgLPS), tumor necrosis factor α (TNF-α), interleukin (IL-12), or a cocktail of interleukins 2/4/6 (IL-2/4/6), then will be analyzed using mass cytometry (CyTOF). The gingival tissue collected from 16 ChP patients at baseline will be examined with imaging mass cytometry (IMC). An integrated multiomic approach will be employed to analyze the results from CyTOF and IMC. The results obtained from this study will empower us to explore the synergies of systemic and oral immune mechanisms that contribute to a defined ChP-driven immune milieu while underscoring the effectiveness of standard nonsurgical periodontal treatment.
Location
Arthur A Dugoni School of Dentistry, 155 5th St, San Francisco, CA 94103, USA
Format
Presentation
Cross-Tissue Analysis Demonstrates Oral and Systemic Link in Chronic Periodontitis
Arthur A Dugoni School of Dentistry, 155 5th St, San Francisco, CA 94103, USA
Despite advances in dental care and oral hygiene, the prevalence of chronic periodontitis (ChP) remains high at 47.2% in the U.S. ChP not only leads to severe gum bleeding and tooth loss but also contributes to systemic diseases, including diabetes mellitus, adverse pregnancy outcomes, and cardiovascular diseases. The mechanisms of the connection between ChP and systemic health are still not fully understood. In our phase I study, we demonstrated systemic and cell-specific immune dysfunctions in patients with ChP, which can be temporarily reversed by the local treatment of ChP. For phase II study, we aim to 1) validate our findings with larger cohorts across wider time range and 2) investigate ChP oral and systemic immunological interaction by a multiplex cross-tissue analysis. Whole-blood samples from 16 patients with ChP and 12 healthy controls without ChP were collected at baseline (n=28), 3 weeks post-ChP treatment (n= 25), and 3 months post-ChP treatment (n= 25) in the Bell Dental Center (San Leandro, CA). The blood samples were left unstimulated or stimulated with Porphyromonas gingivalis lipopolysaccharide (PgLPS), tumor necrosis factor α (TNF-α), interleukin (IL-12), or a cocktail of interleukins 2/4/6 (IL-2/4/6), then will be analyzed using mass cytometry (CyTOF). The gingival tissue collected from 16 ChP patients at baseline will be examined with imaging mass cytometry (IMC). An integrated multiomic approach will be employed to analyze the results from CyTOF and IMC. The results obtained from this study will empower us to explore the synergies of systemic and oral immune mechanisms that contribute to a defined ChP-driven immune milieu while underscoring the effectiveness of standard nonsurgical periodontal treatment.
Comments/Acknowledgements
Presentation Category: Research